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All lanes : Anti-ICAM1 (phospho Y512) antibody (ab51033) at 1/500 dilution
Lane 1 : Hela cells treated with TNF alpha with immunizing peptide
Lane 2 : Hela cells treated with TNF alpha
Predicted band size : 58 kDa
Observed band size : 96 kDa (why is the actual band size different from the predicted?)
Western blot using ab51033 at a dilution of 1/500 and Hela cells treated with TNF-α at 20ng/ml for 5 minutes.
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