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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-ICAM1 (phospho Y512) antibody
See all ICAM1 products (46) ...
Description
Rabbit polyclonal to ICAM1 (phospho Y512)
Specificity
The antibody detects endogenous levels of ICAM-1 only when phosphorylated at tyrosine 512.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
The antiserum was produced against synthesised phosphopeptide derived from ICAM-1 around the phosphorylation site of tyrosine 512 (K-K-YP-R-L)(Human)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Western blot - ICAM1 (phospho Y512) antibody (ab51033)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 96 kDa (predicted molecular weight: 58 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BB 2 antibody
- BB2 antibody
- CD 54 antibody
see all
Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Anti-ICAM1 (phospho Y512) antibody images:
Western blot - ICAM1 (phospho Y512) antibody (ab51033)
All lanes : Anti-ICAM1 (phospho Y512) antibody (ab51033) at 1/500 dilution
Lane 1 : Hela cells treated with TNF alpha with immunizing peptide
Lane 2 : Hela cells treated with TNF alpha
Predicted band size : 58 kDa
Observed band size : 96 kDa (why is the actual band size different from the predicted?)
Western blot using ab51033 at a dilution of 1/500 and Hela cells treated with TNF-α at 20ng/ml for 5 minutes.
References for Anti-ICAM1 (phospho Y512) antibody (ab51033)
ab51033 has not yet been referenced specifically in any publications.
Publishing research using ab51033? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"