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- Primary antibodies
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Product name
ICAM1 protein (Human)
See all ICAM1 products (3) ...
Protein description
Recombinant fragment, corresponding to amino acids 28-480 of Human ICAM1 (Extracellular domain); 453 amino acids, 49.5 kDa. Swiss ID = P05362.
Expression host
CHO
Properties
Purity
> 95% by SDS-PAGE
Purification notes
Purity is greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Form
Lyophilised
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Concentration
Concentration information loading...
Sequence notes
QTSVSPSKV ILPRGGSVLV TCSTSCDQPK LLGIETPLPK KELLLPGNNR KVYELSNVQE DSQPMCYSNC PDGQSTAKTF LTVYWTPERV ELAPLPSWQP VGKNLTLRCQ VEGGAPRANL TVVLLRGEKE LKREPAVGEP AEVTTTVLVR RDHHGANFSC RTELDLRPQG LELFENTSAP YQLQTFVLPA TPPQLVSPRV LEVDTQGTVV CSLDGLFPVS EAQVHLALGD QRLNPTVTYG NDSFSAKASV SVTAEDEGTQ RLTCAVILGN QSQETLQTVT IYSFPAPNVI LTKPEVSEGT EVTVKCEAHP RAKVTLNGVP AQPLGPRAQL LLKATPEDNG RSFSCSATLE VAGQLIHKNQ TRELRVLYGP RLDERDCPGN WTWPENSQQT PMCQAWGNPL PELKCLKDGT FPLPIGESVT VTRDLEGTYL CRARSTQGEV TRKVTVNVLS PRYE
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Sandwich ELISA - ICAM1 protein (Human) (ab82125)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab82125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution.
Monomeric glycosylated ICAM1 migrates at an apparent molecular weight of approximately 72.0-80.0 kDa by SDS-PAGE analysis under reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
ICAM1 protein (Human) images:
Sandwich ELISA - ICAM1 protein (Human) (ab82125)
Standard curve for ICAM1 (Analyte: ab82125); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab2213 at 5µg/ml and Detector Antibody ab7815 at 0.5µg/ml.
References for ICAM1 protein (Human) (ab82125)
ab82125 has not yet been referenced specifically in any publications.
Publishing research using ab82125? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"