![Flow Cytometry / FACS - ICAM2 antibody [CBR-IC2/2] - Azide free (ab35045) image](#ab35045.gif)
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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-ICAM2 antibody [CBR-IC2/2]
See all ICAM2 products (15) ...
Description
Mouse monoclonal [CBR-IC2/2] to ICAM2
Specificity
Recognizes human CD102, also known as intercellular adhesion molecule 2 (ICAM 2).
Tested applications
Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
Cross reactivity
Reacts with
Human
Immunogen
Transfected COS cells expressing ICAM 2 cDNA (Human).
Positive control
Human peripheral blood lymphocytes
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Endotoxin Level: <0.01 EU/ug
Primary antibody notes
Clone CBR-IC2/2 is reported to inhibit interactions between CD102 and LFA1.
Clonality
Monoclonal
Clone number
CBR-IC2/2
Myeloma
P3x63-Ag8.653
Isotype
IgG2a
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Cell Type Markers >> CD >> Adhesion
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Flow Cytometry / FACS - ICAM2 antibody [CBR-IC2/2] - Azide free (ab35045)
![Flow Cytometry / FACS - ICAM2 antibody [CBR-IC2/2] - Azide free (ab35045) image](/ps/datasheet/Images/35/ab35045/ab35045.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab35045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/100(Use 10µl of this concentration to label 106 cells in 100µl.)
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution. (PubMed: 1676048Inhibits interactions between CD102 and LFA1.)
Target
Relevance
ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Cellular localization
Membrane; Single-pass type I membrane protein
Alternative names
- Intercellular adhesion molecule 2 antibody
- CD102 antibody
- CD102 antigen antibody
see all
Database links
- Entrez Gene: 3384 Human
- Omim: 146630 Human
- SwissProt: P13598 Human
Anti-ICAM2 antibody [CBR-IC2/2] images:
Flow Cytometry / FACS - ICAM2 antibody [CBR-IC2/2] - Azide free (ab35045)
Staining of human peripheral blood lymphocytes probed with ab35045 at 1/100 dilution.
References for Anti-ICAM2 antibody [CBR-IC2/2] (ab35045)
This product has been referenced in:
- Thümmler Ket al. Immune regulation by peripheral suppressor T cells induced upon homotypic T cell/T cell interactions. J Leukoc Biol 88:1041-50 (2010). Neutralising; Human.Read more (PubMed: 20651300) »
- Tran DQet al. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. J Immunol 182:2929-38 (2009).Read more (PubMed: 19234188) »
See all 4 publications for this product
Publishing research using ab35045? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"