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Overview

  • Product nameICAM3 Human ELISA KitSee all ICAM3 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 19.75ng/ml 0.172 0.86%
    Sample B 8 2.83ng/ml 0.099 3.49%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 36 19.51ng/ml 0.391 1.99%
    Sample B 36 2.71ng/ml 0.176 6.27%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.3 ng/ml
  • Range
    0.62 ng/ml - 20 ng/ml
  • Assay time
    1h 40m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human ICAM3 ELISA is to be used for the in-vitro quantitative determination of soluble Inter Cellular Adhesion Molecule 3 (ICAM3) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human ICAM3. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The ICAM3 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens and unknowns are pipetted into these wells. During the first incubation, the ICAM3 antigen and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM3 present in the samples.

     

    Recognizes both natural and recombinant human ICAM3

    Range: 0.62 ng/ml - 20 ng/ml

    Sensitivity <0.3 ng/ml

  • Notes

    Safety Notes:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml
    200X Washing Buffer White 1 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml
    Biotinylated anti-sICAM-3 (CD50) Red 1 x 0.4ml
    Chromogen TMB 1 x 11ml
    HRP Diluent Red 1 x 23ml
    ICAM3 Control Silver 2 vials
    ICAM3 precoated microtiter plates 8 units
    ICAM3 Standard Yellow 2 vials
    Stop Reagent Black 1 x 11ml
    Streptavidin-HRP 2 x 5µl
  • Research Areas
  • RelevanceICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
    • Alternative names
        CD50 CD50 antigenCDW50
        ICAM 3 ICAM RICAMRIntercellular adhesion molecule 3Intercellular adhesion molecule 3 precursor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46024 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      ICAM3 Human ELISA Kit images

      • ELISA - Human ICAM3 ELISA Kit (ab46024)
        ELISA - Human ICAM3 ELISA Kit (ab46024)
        Typical ICAM3 standard curve ranging from 0.612 to 20 ng/mL

      References for ICAM3 Human ELISA Kit (ab46024)

      ab46024 has not yet been referenced specifically in any publications.

      Publishing research using ab46024 ? Please let us know so that we can cite the reference in this datasheet

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      What is the expiration date on the following product/lot?:

      ICAM3 Human ELISA Kit (ab46024) lot #GR8821-3

      Thank you for contacting us.

      The expiration date that we assigned for that lot is October 2013. However, our guarantee is in effect for only 6 months after the purchase of the kit, so I recommend contacting us immediately if you have any issues...

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      Is it necessary to use a glass vial for diluting the biotinylated antibody?
      Can plastic vial be used as well?

      Thank you for your patience.

      The lab let me know that it is not necessary to use glass vials. Plastic vials suit perfectly. The crucial point is cleanness.

      I hope this information is helpful to you. Please do not hesitate to contact us i...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"