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Flow Cytometry / FACS - ICAM3 antibody [B-R1] (ab47030)
![Flow Cytometry / FACS - ICAM3 antibody [B-R1] (ab47030)](/ps/datasheet/images/47030.gif)
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Product Name
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ICAM3 antibody [B-R1]
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See all ICAM3 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B-R1] to ICAM3
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Immunogen
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Tissue / cell preparation of U266 cell line (Human)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Flow Cyt, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 10µl for 106 cells or 100µl of whole blood.
IP: Use 10µl for 106 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; Type I membrane protein.
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Research areas
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Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
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Relevance
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All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Upon stimulation by a physiological stimulus it becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B-R1
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Myeloma
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x63-Ag8.653
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Isotype
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IgG1
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Purity
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Ion Exchange Chromatography
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICAM3 antibody [B-R1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ICAM3 antibody [B-R1] images, references, products related to ab47030 and other tools.
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ICAM3 antibody [B-R1] images:
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 Flow Cytometry / FACS - ICAM3 antibody [B-R1] (ab47030)
A typical staining pattern with the B-R1 monoclonal antibody of lymphocytes
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Search PubMed (MEDLINE) for references to ICAM3
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ICAM3 antibody [B-R1] - more information
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Customer reviews (feedback) regarding ICAM3 antibody [B-R1] |
Customer FAQs regarding ICAM3 antibody [B-R1] |
Protocols for ICAM3 antibody [B-R1] |
Price and availability of products related to ICAM3 antibody [B-R1] |
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ICAM3 antibody [B-R1] - related products:
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ICAM3 antibody [B-R1] - other tools:
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Contact Abcam with a Technical Enquiry about ICAM3 antibody [B-R1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry / FACS - ICAM3 antibody [B-R1] (ab47030)](/index.html?datasheet=47030#47030.gif)
