You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
- Primary antibodies
- Secondary antibodies
-
Proteins & Peptides
-
A-Z
- Full length proteins
- Partial proteins
- Peptides
-
Enzymes
- MMPs
- Caspases
- Kinases and transferases
- Signal transduction related
-
Cytokines and growth factors
- Cytokines
- Growth factors by family
-
Human proteins
- Human expressed
- Barley expressed
- Wheat expressed
- Fc fusion proteins
- Ig proteins
-
His-tagged Proteins by research area
- Cancer
- Cardiovascular
- Epigenetics & Nuclear Signaling
- Cell Biology
- Immunology
- Neuroscience
- Signal Transduction
- Stem Cells
-
Microbiology and virology
- All microbiology and viral proteins
- DNA virus proteins
- RNA viral proteins
- All Hepatitis virus proteins
- HIV proteins
- HSV, Measles and Influenza proteins
- Bacterial toxins and chaperones
-
Resources
- Advantages of expression in barley
-
-
ELISA Kits
-
A-Z
- ELISA Kits
- ELIPAIR Kits
- ELISPOT Kits
- FLUOROSPOT Kits
-
ELISA development
- Matched antibody pairs
-
Resources
- Immunoassay technical know-how
- ELISA and ELIPAIR
- ELISPOT and FLUOROSPOT
-
ELISA Kits by protein family
- Adaptive immune components
- Adhesion molecules
- Angiogenic factors
- Apoptosis markers
- Atherosclerotic proteins
- Blood cell antigens
- Cancer metabolism proteins
- Cardiovascular proteins
- CD markers
- Cell cycle proteins
- Complement activation
- Cytokines and cytokine receptors
- Developmental proteins
- Granzymes and perforins
- Growth factors/hormones
- Immunoglobulins
- Oncoproteins
- Pathogen specific immunoglobulins
- Protease inhibitors
- Signal transduction proteins
- Sub cellular markers
- Tumor biomarkers
-
-
Assay Kits
-
A-Z
- Apoptosis kits
- Epigenetic kits
- Metabolism kits
- ChIP kits
-
Metabolism kits by analyte
- Alcohol
- Amino acids
- Calcium
- Cell viability and senescence
- Coenzymes and cofactors
- Creatinine
- Inorganic ions
- Intermediary metabolism
- Lipid metabolism
- Nitric oxide
- Phosphates
- Sugars
- Oxidative stress
- Other metabolism assays
-
Apoptosis kits by analyte
- Annexin V
- Calpain
- Caspases
- Cathepsins
- DNA fragmentation
- Transmembrane potential
- Other Apoptosis assays
-
Epigenetic Kits by modification
- DNA modification and methylation
- Histone demethylation
- Histone methylation activity
- Sample Preparation
- Histone methylation quantification
- Histone acetylation and deacetylation
- Histone phosphorylation
- Sumoylation
-
-
Other Kits
-
A-Z
- All kits
-
Antibody labeling
- EasyLink antibody conjugation kits
-
Cell fractionation
- Cell fractionation kits A-Z
-
Cell Staining Kits
- CytoPainter kits
-
-
Biochemicals
A-Z
- By pharmacology
- By research area
- By chemical type
- By product range
Receptors & Transporters
- Adenosine
- Adrenoceptors
- Cannabinoid
- Catalytic Receptors
- Dopamine
- GABA
- Glutamate
- Glycine
- Histamine
- Imidazoline
- Melatonin
- Muscarinic
- Nicotinic
- Nuclear Receptors
- Opioids
- Peptide Receptors
- Purinergics
- Serotonin
- Transporters
-
Immunodetection
-
IHC Kits
- EXPOSE biotin-free detection kits
- Biotin-Streptavidin detection kits
- Endogenous avidin/biotin blocking systems
- Substrate kits
- Mouse on Mouse detection kit
- BrdU IHC detection kit
-
Secondary antibodies
- Advanced search
-
Reagents and buffers
- Buffers
- Chromogens and enhancers
- Diluents
- Epitope recovery and pretreatments
- Fluorescent ligands
- Mounting media
- PAP pen
- Serum
- Substrates
-
-
Controls
-
A-Z
- Isotype controls
- Loading controls
- Lysates
-
Isotype controls by host species
- Chicken
- Goat
- Hamster (Armenian and Syrian)
- Mouse
- Rabbit
- Rat
- Sheep
-
Lysates by type
- 293T transfected
- Cytoplasmic
- Membrane
- Nuclear
- Tissue
- Total protein
- Whole cell
-
Loading controls
- Loading controls by protein target
-
Product partner
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab10804? Please let us know so that we can cite the reference in this datasheet
ab10804 has been referenced in 2 publications.
- Heasman SJ et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). ICC/IF; Human. PubMed: 20733052
- Gérard A et al. The Par polarity complex regulates Rap1- and chemokine-induced T cell polarization. J Cell Biol 176:863-75 (2007). PubMed: 17353362
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM3 antibody [ICAM3.1] (ab10804)](/ps/datasheet/images/10/ab10804/ICAM3-Primary-antibodies-ab10804-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM3 antibody [ICAM3.1] (ab10804)
Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
![Immunocytochemistry/ Immunofluorescence - ICAM3 antibody [ICAM3.1] (ab10804)](/ps/datasheet/images/10/ab10804/ICAM3-Primary-antibodies-ab10804-2.jpg)
Immunocytochemistry/ Immunofluorescence - ICAM3 antibody [ICAM3.1] (ab10804)
ab10804 staining ICAM3 in Y27632-treated CEM T cells by Immunocytochemistry/ Immunofluorescence.CEM T cells were fixed for 15 minutes with 4% paraformaldehyde. Cells were permeabilized with 0.1% Triton X-100, blocked with 1% BSA, and incubated with ab10804 (1/50), for 1 hour followed by Alexa Fluor 488–conjugated anti–mouse IgG secondary antibodies (1/200).
Image from Heasman SJ et al, J Cell Biol. 2010 Aug 23;190(4):553-63, Fig 5. doi/10.1083/jcb.201002067
0
Events
Talk to us
Special offers
© 1998-2012 Abcam plc. All rights reserved.
United States
0
- A - C
- Albania
- Algeria
- Antigua and Barbuda
- Argentina
- Armenia
- Aruba
- Australia
- Austria
- Azerbaijan
- Bahamas
- Bahrain
- Bangladesh
- Barbados
- Belarus
- Belgium
- Belize
- Bermuda
- Bolivia
- Bosnia and herzegovina
- Botswana
- Brazil
- Brunei darussalam
- Bulgaria
- Cambodia
- Cameroon
- Canada
- Cayman islands
- Chile
- China
- Colombia
- Costa rica
- Cote d'ivoire
- Croatia
- Cyprus
- Czech Republic
- D - I
- Denmark
- Dominica
- Dominican republic
- Ecuador
- Egypt
- El salvador
- Estonia
- Ethiopia
- Finland
- France
- French Guiana
- Gabon
- Gambia
- Georgia
- Germany
- Ghana
- Greece
- Grenada
- Guadeloupe
- Guam
- Guatemala
- Guyana
- Haiti
- Honduras
- Hong Kong
- Hungary
- Iceland
- India
- Indonesia
- Ireland
- Israel
- Italy
- J - O
- Jamaica
- Japan
- Jordan
- Kazakhstan
- Kenya
- Korea, Rep of
- Kuwait
- Kyrgystan
- Latvia
- Lebanon
- Lithuania
- Luxembourg
- Macau
- Macedonia
- Madagascar
- Malawi
- Malaysia
- Malta
- Martinique
- Mexico
- Monaco
- Morocco
- Nepal
- Netherlands
- Netherlands Ant
- New caledonia
- New Zealand
- Nicaragua
- Nigeria
- Norway
- Oman
Call 01223 696 000 or contact us