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Products:Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Overview
Product name
Anti-ICAM3 antibody
See all ICAM3 products (12) ...
Description
Rabbit polyclonal to ICAM3
Specificity
Detects total ICAM3.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic non-phosphopeptide derived from human ICAM3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
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Western blot - ICAM3 antibody (ab61133)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
Cellular localization
Membrane.
Target information above from: UniProt accessionP32942
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 50 antibody
- CD50 antibody
- CD50 antigen antibody
see all
Database links
- Entrez Gene: 3385 Human
- Omim: 146631 Human
- SwissProt: P32942 Human
- Unigene: 353214 Human
- Unigene: 654563 Human
Anti-ICAM3 antibody images:
Western blot - ICAM3 antibody (ab61133)
All lanes : Anti-ICAM3 antibody (ab61133) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells
treated with TNF-a (20ng/ml, 5mins)
Lane 2 : extracts from NIH/3T3 cells
treated with TNF-a (20ng/ml, 5mins) with immunizing peptide
Predicted band size : 59 kDa
Observed band size : 59 kDa
References for Anti-ICAM3 antibody (ab61133)
ab61133 has not yet been referenced specifically in any publications.
Publishing research using ab61133? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"