|
|
|
|
Western blot - ICAM3 antibody (ab61133)
(enlarge)
|
|
|
|
|
Product Name
|
ICAM3 antibody
|
| |
See all ICAM3 antibodies (13)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to ICAM3
|
|
Immunogen
|
Synthetic non-phosphopeptide derived from human ICAM3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
Detects total ICAM3.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cell Membrane; Type I membrane protein.
|
|
Research areas
|
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
|
|
Relevance
|
All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Upon stimulation by a physiological stimulus it becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICAM3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for ICAM3 antibody images, references, products related to ab61133 and other tools.
|
| |
ICAM3 antibody images:
|
 Western blot - ICAM3 antibody (ab61133)
All lanes : ICAM3 antibody (ab61133) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells
treated with TNF-a (20ng/ml, 5mins)
Lane 2 : extracts from NIH/3T3 cells
treated with TNF-a (20ng/ml, 5mins) with immunizing peptide
Predicted band size : 59 kDa
Observed band size : 59 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to ICAM3
|
| |
ICAM3 antibody - more information
|
Customer reviews (feedback) regarding ICAM3 antibody |
Customer FAQs regarding ICAM3 antibody |
Protocols for ICAM3 antibody |
Price and availability of products related to ICAM3 antibody |
| |
ICAM3 antibody - related products:
|
|
|
| |
ICAM3 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about ICAM3 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
