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Western blot - ICAM5 antibody (ab85191)

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Product Name
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ICAM5 antibody
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See all ICAM5 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ICAM5
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Immunogen
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Synthetic peptide corresponding to a region within the N terminal amino acids 72-121 (RRNGTQRGLR WLARQLVDIR EPETQPVCFF RCARRTLQAR GLIRTFQRPD) of human ICAM5 (NP_003250).
BLAST 'RRNGTQRGLR WLARQLVDIR EPETQPVCFF RCARRTLQAR GLIRTFQRPD ' with or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cell lysate
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Cellular localization
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Membrane; single-pass type I membrane protein
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins
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Relevance
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ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None Constituents: 2% Sucrose, PBS
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Purification notes
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ab85191 is purified by peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICAM5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ICAM5 antibody images, references, products related to ab85191 and other tools.
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ICAM5 antibody images:
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Western blot - ICAM5 antibody (ab85191)
ICAM5 antibody (ab85191) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 97 kDa Observed band size : 97 kDa Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
6-18% gel
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Search PubMed (MEDLINE) for references to ICAM5
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ICAM5 antibody - more information
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Customer reviews (feedback) regarding ICAM5 antibody |
Customer FAQs regarding ICAM5 antibody |
Protocols for ICAM5 antibody |
Price and availability of products related to ICAM5 antibody |
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ICAM5 antibody - related products:
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ICAM5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ICAM5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"