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Western blot - ICAM5 antibody (ab85191)
Western blot - ICAM5 antibody (ab85191)
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Product Name 

ICAM5 antibody

 

See all ICAM5 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ICAM5

Immunogen 

Synthetic peptide corresponding to a region within the N terminal amino acids 72-121 (RRNGTQRGLR WLARQLVDIR EPETQPVCFF RCARRTLQAR GLIRTFQRPD) of human ICAM5 (NP_003250).

BLAST 'RRNGTQRGLR WLARQLVDIR EPETQPVCFF RCARRTLQAR GLIRTFQRPD ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293T cell lysate

Cellular localization 

Membrane; single-pass type I membrane protein

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins

Relevance 

ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

 

Alternative names ICAM5 antibody (ab85191)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

7087    (Human)
15898    (Mouse)
313785    (Rat)

Omim

    

601852    (Human)

SwissProt

    

Q9UMF0    (Human)
Q60625    (Mouse)

Unigene

    

465862    (Human)
59765    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

ab85191 is purified by peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICAM5 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ICAM5 antibody images, references, products related to ab85191 and other tools.

 

ICAM5 antibody images:

Product image  Western blot - ICAM5 antibody (ab85191)

Western blot - ICAM5 antibody (ab85191)

ICAM5 antibody (ab85191) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 97 kDa
Observed band size : 97 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

6-18% gel

 
 

Search PubMed (MEDLINE) for references to ICAM5

 

ICAM5 antibody - more information

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ICAM5 antibody - related products:

Products for Western blot

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Lysates

 

Compatible Secondaries for ICAM5 antibody

ab6564

  

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Agarose antibody

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15nm Gold antibody

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5nm Gold antibody

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ab60314

  

Chromeo™ 488 antibody

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Chromeo™ 494 antibody

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Chromeo™ 546 antibody

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Chromeo™ 642 antibody

 

Other ICAM5 Antibodies

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ICAM5 antibody - other tools:

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