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Product Name
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ICEBERG antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ICEBERG
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Immunogen
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Synthetic peptide (Human).
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Reacts with
(species key)
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Hu, Rat
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Specificity
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This antibody reacts predominantly with ICEBERG.
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunoprecipitation: use at a concentration of 3.0 - 5.0 µg / extract from 107 cells.
Western Blotting: use at a concentration of 0.5 - 2.0 µg/ml.
ELISA: use at a concentration of 0.1 - 1.0 µg/ml.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a band of approximately 10 kDa rat brain lysate, which corresponds to the predicted molecular weight of ICEBERG.
Samples were boiled prior to subjecting to a15% SDS-polyacrylamide gel electrophoresis.
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Inhibitors
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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ICEBERG consists essentially only of 1 CARD domain and is an inhibitor of pro-caspase 1 activation. It inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. ICEBERG functions as a competitive antagonist of RIP 2/pro-caspase 1 interactions and inhibits caspase 1-dependent pro-L-1b secretion in mononuclear cells. ICEBERG is induced by proinflammatory stimuli, suggesting that it may be part of a negative feedback loop.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICEBERG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to ICEBERG
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ICEBERG antibody - more information
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Customer reviews (feedback) regarding ICEBERG antibody |
Customer FAQs regarding ICEBERG antibody |
Protocols for ICEBERG antibody |
Price and availability of products related to ICEBERG antibody |
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ICEBERG antibody - related products:
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ICEBERG antibody - other tools:
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Contact Abcam with a Technical Enquiry about ICEBERG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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