![Western blot - ID2 antibody [2457C5a] (ab53545) image](#ab53545.gif)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-ID2 antibody [2457C5a]
See all ID2 products (7) ...
Description
Mouse monoclonal [2457C5a] to ID2
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human ID2
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab53545 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
2457C5a
Isotype
IgG1
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - ID2 antibody [2457C5a] (ab53545)
![Western blot - ID2 antibody [2457C5a] (ab53545) image](/ps/datasheet/Images/53/ab53545/ab53545.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 15 kDa).
Dot: Use at an assay dependent dilution.
Target
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Tissue specificity
Highly expressed in early fetal tissues, including those of the central nervous system.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Developmental stage
Found in most early fetal tissues but not in the corresponding mature tissues.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ02363
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- Class B basic helix-loop-helix protein 26 antibody
see all
Database links
- Entrez Gene: 3398 Human
- Omim: 600386 Human
- SwissProt: Q02363 Human
- Unigene: 180919 Human
Anti-ID2 antibody [2457C5a] images:
Western blot - ID2 antibody [2457C5a] (ab53545)
Anti-ID2 antibody [2457C5a] (ab53545) + immunised recombinant protein
Predicted band size : 15 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-ID2 antibody [2457C5a] (ab53545)
ab53545 has not yet been referenced specifically in any publications.
Publishing research using ab53545? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"