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Western blot - ID2 antibody (ab85990)
(enlarge)
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Product Name
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ID2 antibody
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See all ID2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ID2
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Immunogen
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3 synthetic peptides (Human) conjugated to KLH
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 15 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Recombinant protein produced in an induced culture of E.coli.
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, and may play a role in negatively regulating cell differentiation. Members of the ID family are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. They inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner, by suppressing their heterodimerization partners through the HLH domains.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ID2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ID2 antibody images, references, products related to ab85990 and other tools.
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ID2 antibody images:
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 Western blot - ID2 antibody (ab85990)
All lanes : ID2 antibody (ab85990) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size : 15 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Predicted molecular weight of Recombinant ID2：29 kDa (15 kDa + another 14 kDa for the tag).
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Search PubMed (MEDLINE) for references to ID2
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ID2 antibody - more information
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Customer reviews (feedback) regarding ID2 antibody |
Customer FAQs regarding ID2 antibody |
Protocols for ID2 antibody |
Price and availability of products related to ID2 antibody |
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ID2 antibody - related products:
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ID2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ID2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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