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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-ID2 antibody
See all ID2 products (7) ...
Description
Rabbit polyclonal to ID2
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
3 synthetic peptides (Human) conjugated to KLH
Positive control
Recombinant protein produced in an induced culture of E.coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - ID2 antibody (ab85990)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 29 kDa (predicted molecular weight: 15 kDa).
Target
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Tissue specificity
Highly expressed in early fetal tissues, including those of the central nervous system.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Developmental stage
Found in most early fetal tissues but not in the corresponding mature tissues.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ02363
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- Class B basic helix-loop-helix protein 26 antibody
see all
Database links
- Entrez Gene: 3398 Human
- Entrez Gene: 15902 Mouse
- Omim: 600386 Human
- SwissProt: Q02363 Human
- SwissProt: P41136 Mouse
- Unigene: 180919 Human
- Unigene: 34871 Mouse
Anti-ID2 antibody images:
Western blot - ID2 antibody (ab85990)
All lanes : Anti-ID2 antibody (ab85990) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size : 15 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Predicted molecular weight of Recombinant ID2：29 kDa (15 kDa + another 14 kDa for the tag).
References for Anti-ID2 antibody (ab85990)
ab85990 has not yet been referenced specifically in any publications.
Publishing research using ab85990? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"