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Products:Cell Biology >> Apoptosis >> Nucleus >> Other
Overview
Product name
Anti-ID3 antibody
See all ID3 products (8) ...
Description
Rabbit polyclonal to ID3
Tested applications
IHC-Fr, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to C-terminal of human ID3.
Epitope
C-terminal.
Positive control
Human breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Nucleus >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20460439)
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: 1/50Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
Tissue specificity
Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
Database links
- Entrez Gene: 3399 Human
- Entrez Gene: 15903 Mouse
- Omim: 600277 Human
- SwissProt: Q02535 Human
- SwissProt: P41133 Mouse
- Unigene: 76884 Human
- Unigene: 110 Mouse
Anti-ID3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ID3 antibody (ab41834)
Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence-Anti-ID3 antibody(ab41834)
ICC/IF image of ab41834 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-ID3 antibody (ab41834)
This product has been referenced in:
- Gajera CRet al. LRP2 in ependymal cells regulates BMP signaling in the adult neurogenic niche. J Cell Sci 123:1922-30 (2010). IHC-Fr; Mouse.Read more (PubMed: 20460439) »
See 1 publication for this product
Publishing research using ab41834? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"