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Products:Cell Biology >> Apoptosis >> Nucleus >> Other
Overview
Product name
Anti-ID3 antibody
See all ID3 products (8) ...
Description
Rabbit polyclonal to ID3
Specificity
This antibody reacts with ID3
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Cow, Dog, Pig
Immunogen
A region within synthetic peptide: MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR, corresponding to N terminal amino acids 1-50 of Human ID3
MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR
Positive control
Transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Nucleus >> Other
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Western blot - ID3 antibody (ab50876)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/1562500.
WB: Use at a concentration of 1.25 µg/ml. Predicted molecular weight: 13 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
Tissue specificity
Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
Database links
- Entrez Gene: 538690 Cow
- Entrez Gene: 403547 Dog
- Entrez Gene: 3399 Human
- Entrez Gene: 25585 Rat
- Omim: 600277 Human
- SwissProt: Q3T121 Cow
- SwissProt: Q5E981 Cow
- SwissProt: Q712G9 Dog
see all
Anti-ID3 antibody images:
Western blot - ID3 antibody (ab50876)
Anti-ID3 antibody (ab50876) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 13 kDa
References for Anti-ID3 antibody (ab50876)
ab50876 has not yet been referenced specifically in any publications.
Publishing research using ab50876? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"