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Products:Cell Biology >> Apoptosis >> Nucleus >> Other
Overview
Product name
Anti-ID3 antibody
See all ID3 products (8) ...
Description
Mouse monoclonal to ID3
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVV, corresponding to amino acids 1-84 of Human ID3
MKALSPVRGC YEAVCCLSER SLAIARGRGK GPAAEEPLSL LDDMNHCYSR LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVV
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Light chain type
kappa
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Nucleus >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 13 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
Tissue specificity
Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
Database links
- Entrez Gene: 3399 Human
- Omim: 600277 Human
- SwissProt: Q02535 Human
- Unigene: 76884 Human
Anti-ID3 antibody images:
Western blot - ID3 antibody (ab55269)
ID3 antibody (ab55269) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence-ID3 antibody(ab55269)
ab55269 at 10 ug/ml staining ID3 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-ID3 antibody (ab55269)
ab55269 has not yet been referenced specifically in any publications.
Publishing research using ab55269? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"