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Immunohistochemistry (Formalin fixed sections) - ID4 antibody (ab20988)
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Product Name
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ID4 antibody
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See all ID4 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ID4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ID4.
(Peptide available as ab23374.)
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Reacts with
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Ms
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Predicted to react
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Hu, Rat, Dog
(Due to sequence homology)
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Tested applications
(see key)
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IHC-FoFr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution.
IHC-FoFr: 1/200 - 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse prostate tissue lysate
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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Transcription factors containing a basic helix loop helix (bHLH) motif regulate the expression of tissue specific genes in a number of mammalian and insect systems. DNA binding activity of the bHLH proteins is dependent on formation of homo and/or heterodimers. Dominant negative HLH proteins (encoded by Id related genes) also contain the HLH dimerization domain but lack the DNA binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (referenced from entrez gene).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ID4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ID4 antibody images, references, products related to ab20988 and other tools.
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ID4 antibody images:
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 Immunohistochemistry (Formalin fixed sections) - ID4 antibody (ab20988)
ab20988 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) prostate. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG. Shunyou Wang, Ph.D.
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Search PubMed (MEDLINE) for references to ID4
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ID4 antibody - more information
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Customer reviews (feedback) regarding ID4 antibody |
Customer FAQs regarding ID4 antibody |
Protocols for ID4 antibody |
Price and availability of products related to ID4 antibody |
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ID4 antibody - related products:
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ID4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ID4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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