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Anti-ID4 antibody (ab49261)

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Overview

Product name

Anti-ID4 antibody
See all ID4 products (2) ...

Description

Rabbit polyclonal to ID4

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Dog, Pig

Immunogen

A reigon within synthetic peptide: MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC, corresponding to N terminal amino acids 1-50 of Human ID4

MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HepG2 cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH

  • Western blot - ID4 antibody (ab49261)Western blot - ID4 antibody (ab49261) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP47928 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • bHLHb27 antibody
  • Class B basic helix-loop-helix protein 27 antibody
  • DNA binding protein inhibitor ID 4 antibody
  • DNA binding protein inhibitor ID4 antibody
  • DNA-binding protein inhibitor ID-4 antibody
  • ID 4 antibody
  • Id4 antibody
  • ID4_HUMAN antibody
  • IDB4 antibody
  • Inhibitor of DNA binding 4 antibody
  • Inhibitor of DNA binding 4 dominant negative helix loop helix protein antibody
see all

Database links

see all

Anti-ID4 antibody images:

  Western blot - ID4 antibody (ab49261)

Western blot - ID4 antibody (ab49261)

Anti-ID4 antibody (ab49261) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG 1/50000-1/100000

Predicted band size : 18 kDa
Observed band size : 18 kDa

References for Anti-ID4 antibody (ab49261)

This product has been referenced in:

  • Melcher Ret al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32:636-42 (2011). IHC-P; Human.Read more (PubMed: 21297112) »

See 1 publication for this product

Publishing research using ab49261? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"