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Western blot - ID4 antibody (ab49261)
(enlarge)
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Product Name
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ID4 antibody
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See all ID4 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ID4
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Immunogen
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A reigon within synthetic peptide: MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC, corresponding to N terminal amino acids 1-50 of Human ID4
BLAST 'MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre: 1/1,562,500.
WB: Use at a concentration of 1.25 µg/ml. Predicted molecular weight: 18 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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Transcription factors containing a basic helix loop helix (bHLH) motif regulate the expression of tissue specific genes in a number of mammalian and insect systems. DNA binding activity of the bHLH proteins is dependent on formation of homo and/or heterodimers. Dominant negative HLH proteins (encoded by Id related genes) also contain the HLH dimerization domain but lack the DNA binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (referenced from entrez gene).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 2.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ID4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ID4 antibody images, references, products related to ab49261 and other tools.
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ID4 antibody images:
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 Western blot - ID4 antibody (ab49261)
ID4 antibody (ab49261) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG 1/50000-1/100000
Predicted band size : 18 kDa
Observed band size : 18 kDa
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Search PubMed (MEDLINE) for references to ID4
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ID4 antibody - more information
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Customer reviews (feedback) regarding ID4 antibody |
Customer FAQs regarding ID4 antibody |
Protocols for ID4 antibody |
Price and availability of products related to ID4 antibody |
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ID4 antibody - related products:
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ID4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ID4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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