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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-ID4 antibody
See all ID4 products (2) ...
Description
Rabbit polyclonal to ID4
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Dog, Pig, Zebrafish
Immunogen
Synthetic peptide from within a region of internal sequence amino acids 71-120 (YSRLRRLVPT IPPNKKVSKV EILQHVIDYI LDLQLALETH PALLRQPPPP) of Human ID4 (NP_001537)
Positive control
293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - ID4 antibody (ab86113)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 18 kDa.
Target
Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP47928
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb27 antibody
- Class B basic helix-loop-helix protein 27 antibody
- DNA binding protein inhibitor ID 4 antibody
see all
Database links
- Entrez Gene: 374150 Chicken
- Entrez Gene: 3400 Human
- Entrez Gene: 15904 Mouse
- Omim: 600581 Human
- SwissProt: Q90X13 Chicken
- SwissProt: P47928 Human
- SwissProt: P41139 Mouse
- SwissProt: Q544D2 Mouse
see all
Anti-ID4 antibody images:
Western blot - ID4 antibody (ab86113)
Anti-ID4 antibody (ab86113) at 1 µg/ml (5% skim milk/PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 10-20%
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 18 kDa
Observed band size : 18 kDa
References for Anti-ID4 antibody (ab86113)
ab86113 has not yet been referenced specifically in any publications.
Publishing research using ab86113? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"