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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-IDH2 antibody
See all IDH2 products (5) ...
Description
Mouse monoclonal to IDH2
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human, Recombinant Fragment
Immunogen
Recombinant fragment: HYREHQKGRP TSTNPIASIF AWTRGLEHRG KLDGNQDLIR FAQMLEKVCV ETVESGAMTK DLAGCIHGLS NVKLNEHFLN TTDFLDTIKS NLDRALGR, corresponding to amino acids 354-452 of Human IDH2
HYREHQKGRP TSTNPIASIF AWTRGLEHRG KLDGNQDLIR FAQMLEKVCV ETVESGAMTK DLAGCIHGLS NVKLNEHFLN TTDFLDTIKS NLDRALGR
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 3 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 1 - 5 µg/ml.(This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.)
Target
Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Involvement in disease
Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2) [MIM:613657]. D2HGA2 is a neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine.
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP48735
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ICD-M antibody
- IDH antibody
- IDH2 antibody
see all
Database links
- Entrez Gene: 3418 Human
- Omim: 147650 Human
- SwissProt: P48735 Human
- Unigene: 596461 Human
Anti-IDH2 antibody images:
IHC-P - IDH2 antibody (ab55271)
IDH2 antibody (ab55271) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - Anti-IDH2 antibody (ab55271)
Western blot against tagged recombinant protein immunogen using ab55271 IDH2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Immunocytochemistry/ Immunofluorescence-IDH2 antibody(ab55271)
ICC/IF image of ab55271 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55271, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-IDH2 antibody (ab55271)
All lanes : Anti-IDH2 antibody (ab55271)
Lane 1 : IDH2 in transfected 293T cell line
Lane 2 : Non-transfected lysate
The blocking agent used is 5% milk.
References for Anti-IDH2 antibody (ab55271)
This product has been referenced in:
- Wise DRet al. Hypoxia promotes isocitrate dehydrogenase-dependent carboxylation of a-ketoglutarate to citrate to support cell growth and viability. Proc Natl Acad Sci U S A : (2011). WB; Human.Read more (PubMed: 22106302) »
- Ward PSet al. The Common Feature of Leukemia-Associated IDH1 and IDH2 Mutations Is a Neomorphic Enzyme Activity Converting alpha-Ketoglutarate to 2-Hydroxyglutarate. Cancer Cell : (2010). WB; Human.Read more (PubMed: 20171147) »
See all 3 publications for this product
Publishing research using ab55271? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"