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Anti-IDH2 antibody (ab55271)

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Overview

Product name

Anti-IDH2 antibody
See all IDH2 products (5) ...

Description

Mouse monoclonal to IDH2

Tested applications

IHC-P, ICC/IF, WBmore details

Cross reactivity

Reacts with

Human, Recombinant Fragment

Immunogen

Recombinant fragment: HYREHQKGRP TSTNPIASIF AWTRGLEHRG KLDGNQDLIR FAQMLEKVCV ETVESGAMTK DLAGCIHGLS NVKLNEHFLN TTDFLDTIKS NLDRALGR, corresponding to amino acids 354-452 of Human IDH2

HYREHQKGRP TSTNPIASIF AWTRGLEHRG KLDGNQDLIR FAQMLEKVCV ETVESGAMTK DLAGCIHGLS NVKLNEHFLN TTDFLDTIKS NLDRALGR

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG1

Light chain type

kappa

  • IHC-P - IDH2 antibody (ab55271)IHC-P - IDH2 antibody (ab55271) image (enlarge)

  • Western blot - Anti-IDH2 antibody (ab55271)Western blot - Anti-IDH2 antibody (ab55271) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-IDH2 antibody(ab55271)Immunocytochemistry/ Immunofluorescence-IDH2 antibody(ab55271) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab55271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 3 µg/ml

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 10 µg/ml

  • ShowHide2 Images

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 5 µg/ml.(This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.)

Target

Function

Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

Involvement in disease

Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2) [MIM:613657]. D2HGA2 is a neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Cellular localization

Mitochondrion.

Target information above from: UniProt accessionP48735 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ICD-M antibody
  • IDH antibody
  • IDH2 antibody
  • IDHP_HUMAN antibody
  • IDP antibody
  • Isocitrate dehydrogenase [NADP], mitochondrial antibody
  • mNADP-IDH antibody
  • NADP(+)-specific ICDH antibody
  • Oxalosuccinate decarboxylase antibody
see all

Anti-IDH2 antibody images:

  IHC-P - IDH2 antibody (ab55271)

IHC-P - IDH2 antibody (ab55271)

IDH2 antibody (ab55271) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

  Western blot - Anti-IDH2 antibody (ab55271)

Western blot - Anti-IDH2 antibody (ab55271)

Western blot against tagged recombinant protein immunogen using ab55271 IDH2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

  Immunocytochemistry/ Immunofluorescence-IDH2 antibody(ab55271)

Immunocytochemistry/ Immunofluorescence-IDH2 antibody(ab55271)

ICC/IF image of ab55271 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55271, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Western blot - Anti-IDH2 antibody (ab55271)

Western blot - Anti-IDH2 antibody (ab55271)

All lanes : Anti-IDH2 antibody (ab55271)

Lane 1 : IDH2 in transfected 293T cell line
Lane 2 : Non-transfected lysate


The blocking agent used is 5% milk.

References for Anti-IDH2 antibody (ab55271)

This product has been referenced in:

  • Wise DRet al. Hypoxia promotes isocitrate dehydrogenase-dependent carboxylation of a-ketoglutarate to citrate to support cell growth and viability. Proc Natl Acad Sci U S A : (2011). WB; Human.Read more (PubMed: 22106302) »
  • Ward PSet al. The Common Feature of Leukemia-Associated IDH1 and IDH2 Mutations Is a Neomorphic Enzyme Activity Converting alpha-Ketoglutarate to 2-Hydroxyglutarate. Cancer Cell : (2010). WB; Human.Read more (PubMed: 20171147) »

See all 3 publications for this product

Publishing research using ab55271? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"