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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab84726 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - IDH2 antibody (ab84726)
Anti-IDH2 antibody (ab84726) at 1 µg/ml + Human fetal muscle lysate at 10 µg
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 51 kDa
Observed band size : 51 kDa
Immunocytochemistry/ Immunofluorescence-IDH2 antibody(ab84726)
ICC/IF image of ab84726 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84726, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody (ab84726)
IHC image of ab84726 staining in Hu Colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-IDH2 antibody (ab84726)
All lanes : Anti-IDH2 antibody (ab84726) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish skeletal muscle homogenate (20ug)
Lane 3 : JURKAT (Human T cell lymphoblast-like cell line) Whole Cell Lysate (20ug)
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 51 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
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