Feedback Form

Western blot - IDH2 antibody (ab84726)
Western blot - IDH2 antibody (ab84726)
(enlarge)


Product Name 

IDH2 antibody

 

See all IDH2 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to IDH2

Immunogen 

Synthetic peptide, corresponding to a region within internal sequence amino acids 143-192 (GGTVFREPI ICKNIPRLVP GWTKPITIGR HAHGDQYKAT DFVADRAGTF of Human IDH2 (NP_002159)

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human fetal muscle lysate.

Cellular localization 

Mitochondrial

Research areas 

Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

IDH2 is an NADP-dependent isocitrate dehydrogenase which catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. It is found in the mitochondrian and plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

 

Alternative names IDH2 antibody (ab84726)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3418    (Human)
269951    (Mouse)
361596    (Rat)

Omim

    

147650    (Human)

SwissProt

    

P48735    (Human)
P54071    (Mouse)
P56574    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

ab84726 is purified by a peptide affinity chromatography method.

Form 

Lyophilised
Add 50 µl of distilled water.

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IDH2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for IDH2 antibody images, references, products related to ab84726 and other tools.

 

IDH2 antibody images:

Product image  Western blot - IDH2 antibody (ab84726)

Western blot - IDH2 antibody (ab84726)

IDH2 antibody (ab84726) at 1 µg/ml + Human fetal muscle lysate at 10 µg

Secondary
anti-Rabbit IgG HRP at 1/50000 dilution

Predicted band size : 47 kDa
Observed band size : 47 kDa

 
 

Search PubMed (MEDLINE) for references to IDH2

 

IDH2 antibody - more information

Customer reviews (feedback) regarding IDH2 antibody

Customer FAQs regarding IDH2 antibody

Protocols for IDH2 antibody

Price and availability of products related to IDH2 antibody

 

IDH2 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for IDH2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other IDH2 Antibodies

See all IDH2 antibodies (2)...

 

IDH2 antibody - other tools:

Contact Abcam with a Technical Enquiry about IDH2 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.