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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-IDH3A antibody
See all IDH3A products (4) ...
Description
Rabbit polyclonal to IDH3A
Specificity
This antibody reacts with IDH3A.
Tested applications
ICC/IF, ELISA, WB, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog, Zebrafish
Immunogen
A region within synthetic peptide: IPGDGIGPEI SAAVMKIFDA AKAPIQWEER NVTAIQGPGG KWMIPSEAKE, corresponding to N terminal amino acids 37-86 of Human IDH3A
IPGDGIGPEI SAAVMKIFDA AKAPIQWEER NVTAIQGPGG KWMIPSEAKE
Positive control
Jurkat cell lysate and human skeletal muscle tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
ELISA
ELISA: 1/312500ELISA: 1/312500
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
IP: Use at an assay dependent concentration.
Target
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP50213
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- H IDH alpha antibody
- HIDH alpha antibody
see all
Database links
- Entrez Gene: 282446 Cow
- Entrez Gene: 479066 Dog
- Entrez Gene: 3419 Human
- Entrez Gene: 67834 Mouse
- Entrez Gene: 114096 Rat
- Omim: 601149 Human
- SwissProt: P41563 Cow
- SwissProt: P54836 Dog
see all
Anti-IDH3A antibody images:
Western blot - IDH3A antibody (ab58641)
Anti-IDH3A antibody (ab58641) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa
Immunohistochemistry (Paraffin-embedded sections) - IDH3A antibody (ab58641)
ab58641 at a 4.0µg/ml dilution staining IDH3A in human skeletal muscle cells. Arrows indicate positively labelled skeletal muscle cells.
Western blot - IDH3A antibody (ab58641)
All lanes : Anti-IDH3A antibody (ab58641) at 1/10000 dilution (For 16 hours at 4°C)
Lane 1 : Mouse adipocyte whole cell lysate
Lane 2 : Mouse adipocyte whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 40 kDa
Exposure time : 1 second
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-IDH3A antibody(ab58641)
ICC/IF image of ab58641 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58641, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunoprecipitation - Anti-IDH3A antibody (ab58641)
IDH3A was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5ug of Rabbit polyclonal to IDH3Aand 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58641. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Bands: 38kDa;IDH3A.
References for Anti-IDH3A antibody (ab58641)
This product has been referenced in:
- Ward PSet al. The Common Feature of Leukemia-Associated IDH1 and IDH2 Mutations Is a Neomorphic Enzyme Activity Converting alpha-Ketoglutarate to 2-Hydroxyglutarate. Cancer Cell : (2010). WB; Human.Read more (PubMed: 20171147) »
See 1 publication for this product
Publishing research using ab58641? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"