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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-IDH3A antibody
See all IDH3A products (4) ...
Description
Mouse polyclonal to IDH3A
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Full length Human IDH3A (NP_005521.1)
Positive control
Rat brain tissue lysate, A-431 cell lysate, IDH3A transfected 293T cell lysate and HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 40 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP50213
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- EC 1.1.1.41 antibody
- H IDH alpha antibody
see all
Database links
- Entrez Gene: 3419 Human
- Entrez Gene: 114096 Rat
- Omim: 601149 Human
- SwissProt: P50213 Human
- SwissProt: Q99NA5 Rat
- Unigene: 591110 Human
- Unigene: 95104 Rat
Anti-IDH3A antibody images:
Western blot - IDH3A antibody (ab89153)
Anti-IDH3A antibody (ab89153) at 1 µg/ml + Rat brain tissue lysate at 50 µg
Predicted band size : 40 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Western blot - IDH3A antibody (ab89153)
Anti-IDH3A antibody (ab89153) at 1 µg/ml + A-431 cell lysate at 50 µg
Predicted band size : 40 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Western blot - IDH3A antibody (ab89153)
All lanes : Anti-IDH3A antibody (ab89153) at 1 µg/ml
Lane 1 : IDH3A transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 40 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - IDH3A antibody (ab89153)
ab89153 at 10µg/ml staining IDH3A in HeLa cells by Immunofluorescence.
References for Anti-IDH3A antibody (ab89153)
ab89153 has not yet been referenced specifically in any publications.
Publishing research using ab89153? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"