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Western blot - IDH3A antibody (ab89153)
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Western blot - IDH3A antibody (ab89153)
(enlarge)
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Western blot - IDH3A antibody (ab89153)
(enlarge)
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Product Name
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IDH3A antibody
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See all IDH3A antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to IDH3A
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Immunogen
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Full length Human IDH3A (NP_005521.1)
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat brain tissue lysate, A-431 cell lysate, IDH3A transfected 293T cell lysate and HeLa cells.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IDH3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IDH3A antibody images, references, products related to ab89153 and other tools.
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IDH3A antibody images:
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 Western blot - IDH3A antibody (ab89153)
All lanes : IDH3A antibody (ab89153) at 1 µg/ml
Lane 1 : IDH3A transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 40 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - IDH3A antibody (ab89153)
ab89153 at 10µg/ml staining IDH3A in HeLa cells by Immunofluorescence.
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Search PubMed (MEDLINE) for references to IDH3A
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IDH3A antibody - more information
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Customer reviews (feedback) regarding IDH3A antibody |
Customer FAQs regarding IDH3A antibody |
Protocols for IDH3A antibody |
Price and availability of products related to IDH3A antibody |
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IDH3A antibody - related products:
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IDH3A antibody - other tools:
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Contact Abcam with a Technical Enquiry about IDH3A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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