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Anti-IDH3G antibody (ab67025) at 1/500 dilution + rat brain lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-IDH3G antibody (ab67025) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
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