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Western blot - IDH3G antibody (ab67025)
(enlarge)
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Western blot - IDH3G antibody (ab67025)
(enlarge)
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Product Name
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IDH3G antibody
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See all IDH3G antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to IDH3G
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Immunogen
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Full length protein (Human)
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Brain.
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Cellular localization
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Mitochondrial
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD(+) as the electron acceptor, and is localised to the mitochondrial matrix.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IDH3G antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IDH3G antibody images, references, products related to ab67025 and other tools.
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IDH3G antibody images:
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 Western blot - IDH3G antibody (ab67025)
IDH3G antibody (ab67025) at 1/500 dilution + rat brain lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
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Western blot - IDH3G antibody (ab67025)
All lanes : IDH3G antibody (ab67025) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to IDH3G
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IDH3G antibody - more information
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Customer reviews (feedback) regarding IDH3G antibody |
Customer FAQs regarding IDH3G antibody |
Protocols for IDH3G antibody |
Price and availability of products related to IDH3G antibody |
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IDH3G antibody - related products:
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IDH3G antibody - other tools:
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Contact Abcam with a Technical Enquiry about IDH3G antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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