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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-IDH3G antibody
See all IDH3G products (5) ...
Description
Mouse polyclonal to IDH3G
Tested applications
Cross reactivity
Reacts with
Rat, Human
Immunogen
Full length protein (Human)
Positive control
Brain.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).
Target
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP51553
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- H IDHG antibody
- H-IDHG antibody
see all
Database links
- Entrez Gene: 3421 Human
- Entrez Gene: 25179 Rat
- Omim: 300089 Human
- SwissProt: P51553 Human
- SwissProt: P41565 Rat
- Unigene: 410197 Human
Anti-IDH3G antibody images:
Western blot - IDH3G antibody (ab67025)
Anti-IDH3G antibody (ab67025) at 1/500 dilution + rat brain lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot - IDH3G antibody (ab67025)
All lanes : Anti-IDH3G antibody (ab67025) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
References for Anti-IDH3G antibody (ab67025)
ab67025 has not yet been referenced specifically in any publications.
Publishing research using ab67025? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"