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Western blot - IDH3G antibody (ab67025)
Western blot - IDH3G antibody (ab67025)
(enlarge)


Western blot - IDH3G antibody (ab67025)
Western blot - IDH3G antibody (ab67025)
(enlarge)


Product Name 

IDH3G antibody

 

See all IDH3G antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse polyclonal to IDH3G

Immunogen 

Full length protein (Human)

Reacts with 
(species key)

Hu, Rat

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Brain.

Cellular localization 

Mitochondrial

Research areas 

Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD(+) as the electron acceptor, and is localised to the mitochondrial matrix.

 

Alternative names IDH3G antibody (ab67025)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3421    (Human)
25179    (Rat)

Omim

    

300089    (Human)

SwissProt

    

P51553    (Human)
P41565    (Rat)

Unigene

    

410197    (Human)

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IDH3G antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for IDH3G antibody images, references, products related to ab67025 and other tools.

 

IDH3G antibody images:

Product image  Western blot - IDH3G antibody (ab67025)

Western blot - IDH3G antibody (ab67025)

IDH3G antibody (ab67025) at 1/500 dilution + rat brain lysate at 25 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 43 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)

 

Product image  Western blot - IDH3G antibody (ab67025)

Western blot - IDH3G antibody (ab67025)

All lanes : IDH3G antibody (ab67025) at 1/500 dilution

Lane 1 : Transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to IDH3G

 

IDH3G antibody - more information

Customer reviews (feedback) regarding IDH3G antibody

Customer FAQs regarding IDH3G antibody

Protocols for IDH3G antibody

Price and availability of products related to IDH3G antibody

 

IDH3G antibody - related products:

Products for Western blot

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Lysates

 

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ab60313

  

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Other IDH3G Antibodies

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IDH3G antibody - other tools:

Contact Abcam with a Technical Enquiry about IDH3G antibody

 

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