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Western blot - IDI1 antibody (ab81491)
(enlarge)
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Product Name
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IDI1 antibody
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See all IDI1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to IDI1
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Immunogen
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Synthetic peptide, corresponding to a region within internal sequence amino acids 144-193 (SHPLSNPAEL EESDALGVRR AAQRRLKAEL GIPLEEVPPE EINYLTRIHY) of human IDI1 (NP_004499).
BLAST 'SHPLSNPAEL EESDALGVRR AAQRRLKAEL GIPLEEVPPE EINYLTRIHY ' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:1562500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Cellular localization
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Peroxisome
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Relevance
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IDI1 is a peroxisomally localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). These are substrates for the reactions that ultimately result in the synthesis of cholesterol. There is reduction in IPP isomerase activity in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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Purified by peptide affinity chromatography method.
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Form
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Lyophilised
Add 50 µl of distilled water for a final antibody concentration of 1 mg/ml.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IDI1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IDI1 antibody images, references, products related to ab81491 and other tools.
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IDI1 antibody images:
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 Western blot - IDI1 antibody (ab81491)
IDI1 antibody (ab81491) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 26 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to IDI1
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IDI1 antibody - more information
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Customer reviews (feedback) regarding IDI1 antibody |
Customer FAQs regarding IDI1 antibody |
Protocols for IDI1 antibody |
Price and availability of products related to IDI1 antibody |
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IDI1 antibody - related products:
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IDI1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about IDI1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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