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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-IDI1 antibody
See all IDI1 products (3) ...
Description
Rabbit polyclonal to IDI1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide, corresponding to a region within internal sequence amino acids 144-193 (SHPLSNPAEL EESDALGVRR AAQRRLKAEL GIPLEEVPPE EINYLTRIHY) of human IDI1 (NP_004499).
SHPLSNPAEL EESDALGVRR AAQRRLKAEL GIPLEEVPPE EINYLTRIHY
Positive control
HeLa cell lysate.
Properties
Form
Lyophilised:
Add 50 µl of distilled water for a final antibody concentration of 1 mg/ml.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - IDI1 antibody (ab81491)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:1562500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).
Pathway
Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.
Sequence similarities
Belongs to the IPP isomerase type 1 family.
Contains 1 nudix hydrolase domain.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionQ13907
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- IDI1 antibody
- IDI1_HUMAN antibody
- IPP isomerase 1 antibody
see all
Database links
- Entrez Gene: 3422 Human
- Entrez Gene: 319554 Mouse
- Entrez Gene: 89784 Rat
- Omim: 604055 Human
- SwissProt: Q13907 Human
- SwissProt: P58044 Mouse
- SwissProt: O35760 Rat
- Unigene: 644603 Human
see all
Anti-IDI1 antibody images:
Western blot - IDI1 antibody (ab81491)
Anti-IDI1 antibody (ab81491) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 26 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
References for Anti-IDI1 antibody (ab81491)
ab81491 has not yet been referenced specifically in any publications.
Publishing research using ab81491? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"