Anti-MTIF2 antibody (ab121243)
Key features and details
- Rabbit polyclonal to MTIF2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTIF2 antibody
See all MTIF2 primary antibodies -
Description
Rabbit polyclonal to MTIF2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MTIF2 aa 497-646.
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Positive control
- Human cell line A431, Human plasma lysate, Human nasopharynx tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. -
Tissue specificity
Expressed in all tissues examined. Highest level in skeletal muscle. -
Sequence similarities
Belongs to the IF-2 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 4528 Human
- Omim: 603766 Human
- SwissProt: P46199 Human
- Unigene: 149894 Human
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Alternative names
- IF-2(Mt) antibody
- IF-2Mt antibody
- IF2(mt) antibody
see all
Images
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All lanes : Anti-MTIF2 antibody (ab121243) at 1/250 dilution
Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma lysate
Lane 5 : Human liver lysate
Lane 6 : Human tonsil lysate -
Immunohistochemical staining of paraffin embedded Human nasopharynx tissue, using 1/25 ab121243, shows strong membranous positivity in respiratory epithelial cells.
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Immunofluorescent staining, using 4 µg/ml ab121243, in Human cell line A431 shows positivity in mitochondria. Antibody staining is shown in green.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121243 has not yet been referenced specifically in any publications.