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Anti-IFI16 antibody [IFI-230] (ab50004) at 1.4 µg/ml + Total cell extract of Jurkat cells
Secondary
HRP-Goat anti-Mouse
Predicted band size : 88 kDa
Observed band size : ~75,~80,~85 kDa (why is the actual band size different from the predicted?)
Bands correspond to the three isoforms, A, B and C.
Immunocytochemistry analysis showing nuclear localization of IFI16. Jurkat cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with ab50004 (80 µg/mL) and further developed with Rabbit anti-Mouse IgG-FITC conjugate (Figure A). For nucleus localization, the cells were further treated with DAPI (Figure B).
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