Overview

  • Product nameAnti-IFI16 antibody [IFI-230]See all IFI16 primary antibodies ...
  • Description
    Mouse monoclonal [IFI-230] to IFI16
  • Tested applicationsPurification, WB, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    KDILNPDSSMETSPDF

    , corresponding to C terminal amino acids 768-783 of Human IFI16

  • Positive control
    • Whole extract of the cultured Jurkat cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab50004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration. PubMed: 22691496
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 88 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceInterferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
  • Cellular localizationNucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.
  • Database links
  • Alternative names
    • IFI16 antibody
    • Gamma interferon inducible protein 16 antibody
    • Gamma-interferon-inducible protein Ifi16 antibody
    • IF16_HUMAN antibody
    • Ifi204 antibody
    • IFNGIP1 antibody
    • interferon activated gene 204 antibody
    • Interferon gamma induced protein IFI 16 antibody
    • Interferon gamma inducible protein 16 antibody
    • Interferon-inducible myeloid differentiation transcriptional activator antibody
    • p204 antibody
    • PYHIN2 antibody
    see all

Anti-IFI16 antibody [IFI-230] images

  • Anti-IFI16 antibody [IFI-230] (ab50004) at 1.4 µg/ml + Total cell extract of Jurkat cells

    Secondary
    HRP-Goat anti-Mouse

    Predicted band size : 88 kDa
    Observed band size : ~75,~80,~85 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry analysis showing nuclear localization of IFI16. Jurkat cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with ab50004 (80 µg/mL) and further developed with Rabbit anti-Mouse IgG-FITC conjugate (Figure A). For nucleus localization, the cells were further treated with DAPI (Figure B).

References for Anti-IFI16 antibody [IFI-230] (ab50004)

This product has been referenced in:
  • Kaminski JJ  et al. Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation. J Immunol 191:3876-83 (2013). Read more (PubMed: 23986531) »
  • Brázda V  et al. Preferential binding of IFI16 protein to cruciform structure and superhelical DNA. Biochem Biophys Res Commun 422:716-20 (2012). Human . Read more (PubMed: 22618232) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"