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Anti-IFI16 antibody [IFI-230] (ab50004)

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Overview

Product name

Anti-IFI16 antibody [IFI-230]
See all IFI16 products (3) ...

Description

Mouse monoclonal [IFI-230] to IFI16

Tested applications

WB, IP, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: KDILNPDSSMETSPDF, corresponding to C terminal amino acids 768-783 of Human IFI16

KDILNPDSSM ETSPDF

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Whole extract of the cultured Jurkat cell line

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

IFI-230

Myeloma

NS1

Isotype

IgG1

Research areas

Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

  • Western blot - IFI16 antibody [IFI-230] (ab50004)Western blot - IFI16 antibody [IFI-230] (ab50004) image (enlarge)

  • Immunocytochemistry - IFI16 antibody [IFI-230] (ab50004)Immunocytochemistry - IFI16 antibody [IFI-230] (ab50004) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.

Cellular localization

Nuclear

Alternative names

  • Gamma-interferon-inducible protein Ifi16 antibody
  • Interferon-inducible myeloid differentiation transcriptional activator antibody

Database links

Anti-IFI16 antibody [IFI-230] images:

  Western blot - IFI16 antibody [IFI-230] (ab50004)

Western blot - IFI16 antibody [IFI-230] (ab50004)

Anti-IFI16 antibody [IFI-230] (ab50004) at 1.4 µg/ml + Total cell extract of Jurkat cells

Secondary
HRP-Goat anti-Mouse

Predicted band size : 88 kDa
Observed band size : ~75,~80,~85 kDa (why is the actual band size different from the predicted?)


Bands correspond to the three isoforms, A, B and C.

  Immunocytochemistry - IFI16 antibody [IFI-230] (ab50004)

Immunocytochemistry - IFI16 antibody [IFI-230] (ab50004)

Immunocytochemistry analysis showing nuclear localization of IFI16. Jurkat cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with ab50004 (80 µg/mL) and further developed with Rabbit anti-Mouse IgG-FITC conjugate (Figure A). For nucleus localization, the cells were further treated with DAPI (Figure B).

References for Anti-IFI16 antibody [IFI-230] (ab50004)

ab50004 has not yet been referenced specifically in any publications.

Publishing research using ab50004? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"