IFI16 antibody [IFI-230] (ab50004)

Product Name 

IFI16 antibody [IFI-230]

Product type 

Primary antibodies

Description 

Mouse monoclonal [IFI-230] to IFI16

Immunogen 

Synthetic peptide: KDILNPDSSMETSPDF, corresponding to C terminal amino acids 768-783 of Human IFI16
BLAST 'KDILNPDSSMETSPDF' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 88 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole extract of the cultured Jurkat cell line

Cellular localization 

Nuclear

Research areas 

Cell Biology >> Other Antibodies >> Oxidative Stress
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

Relevance 

Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

IFI-230

Myeloma 

NS1

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.400 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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