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Recombinant fragment: FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF , corresponding to amino acids 630-730 of Human IFI16
Our Abpromise guarantee covers the use of ab55328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.|
|IHC||Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|Purification||Use at an assay dependent concentration. PubMed: 22691496|
This image is courtesy of an Abreview submitted by Jason Dehart
ab55328 staining IFI16 (green) in Human Saos2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% normal goat serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 5% normal goat serum in PBS) for 1 hour at 20°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. DAPI - blue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"