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Anti-IFI16 antibody (ab55328)

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Overview

Product name

Anti-IFI16 antibody
See all IFI16 products (3) ...

Description

Mouse monoclonal to IFI16

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment: FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF , corresponding to amino acids 630-730 of Human IFI16

FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG2b

Light chain type

kappa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328) image (enlarge)

  • Western blot - IFI16 antibody (ab55328)Western blot - IFI16 antibody (ab55328) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab55328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.

Cellular localization

Nuclear

Alternative names

  • Gamma-interferon-inducible protein Ifi16 antibody
  • Interferon-inducible myeloid differentiation transcriptional activator antibody

Anti-IFI16 antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328)

IFI16 antibody (ab55328) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

  Western blot - IFI16 antibody (ab55328)

Western blot - IFI16 antibody (ab55328)

IFI16 antibody (ab55328) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-IFI16 antibody (ab55328)

ab55328 has not yet been referenced specifically in any publications.

Publishing research using ab55328? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"