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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328)

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Western blot - IFI16 antibody (ab55328)

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Product Name
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IFI16 antibody
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See all IFI16 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to IFI16
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Immunogen
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Recombinant fragment: FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF , corresponding to amino acids 630-730 of Human IFI16 BLAST 'FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF ' with or 
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Reacts with
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Hu
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Tested applications
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1-5 µg/ml. IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Other Antibodies >> Oxidative Stress Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors Chromatin and Nuclear Signaling >> Transcription >> Other factors Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53 Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Relevance
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Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IFI16 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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IFI16 antibody images:
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328)
IFI16 antibody (ab55328) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
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Western blot - IFI16 antibody (ab55328)
Predicted band size : 88 kDa
IFI16 antibody (ab55328) at 1ug/lane + HeLa cell lysate at 25ug/lane.
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Search PubMed (MEDLINE) for references to IFI16
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IFI16 antibody - more information
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Customer reviews (feedback) regarding IFI16 antibody |
Customer FAQs regarding IFI16 antibody |
Protocols for IFI16 antibody |
Price and availability of products related to IFI16 antibody |
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IFI16 antibody - other tools:
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Contact Abcam with a Technical Enquiry about IFI16 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"