Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab55328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration.
Purification Use at an assay dependent concentration. PubMed: 22691496

Target

  • RelevanceInterferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
  • Cellular localizationNucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.
  • Database links
  • Alternative names
    • IFI16 antibody
    • Gamma interferon inducible protein 16 antibody
    • Gamma-interferon-inducible protein Ifi16 antibody
    • IF16_HUMAN antibody
    • Ifi204 antibody
    • IFNGIP1 antibody
    • interferon activated gene 204 antibody
    • Interferon gamma induced protein IFI 16 antibody
    • Interferon gamma inducible protein 16 antibody
    • Interferon-inducible myeloid differentiation transcriptional activator antibody
    • p204 antibody
    • PYHIN2 antibody
    see all

Anti-IFI16 antibody images

  • All lanes : Anti-IFI16 antibody (ab55328) at 1/1000 dilution

    Lane 1 : Human sjsa1 whole cell lysate without siRNA to IFI16
    Lane 2 : Human sjsa1 whole cell lysate with siRNA to IFI16

    Lysates/proteins at 30 µg per lane.

    Secondary
    IRDye® 680-conjugated Mouse anti-mouse IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Jason Dehart

    See Abreview

  • ab55328 staining IFI16 (green) in Human Saos2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% normal goat serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 5% normal goat serum in PBS) for 1 hour at 20°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. DAPI - blue.

    See Abreview

  • IFI16 antibody (ab55328) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 88 kDa
    IFI16 antibody (ab55328) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing Jurkat cells stained with ab55328 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55328, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-IFI16 antibody (ab55328)

This product has been referenced in:
  • Kalamvoki M & Roizman B HSV-1 degrades, stabilizes, requires, or is stung by STING depending on ICP0, the US3 protein kinase, and cell derivation. Proc Natl Acad Sci U S A 111:E611-7 (2014). WB ; Mouse . Read more (PubMed: 24449861) »
  • Orzalli MH  et al. Nuclear interferon-inducible protein 16 promotes silencing of herpesviral and transfected DNA. Proc Natl Acad Sci U S A 110:E4492-501 (2013). WB, ICC/IF . Read more (PubMed: 24198334) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step (agent) for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Human Cell (Saos2)
Specification Saos2
Permeabilization Yes - 0.2% Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - whole cell (sjsa1)
Specification sjsa1
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jason Dehart

Verified customer

Submitted Sep 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"