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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-IFI16 antibody
See all IFI16 products (3) ...
Description
Mouse monoclonal to IFI16
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF , corresponding to amino acids 630-730 of Human IFI16
FVNGVFEVHK KNVRGEFTYY EIQDNTGKME VVVHGRLTTI NCEEGDKLKL TCFELAPKSG NTGELRSVIH SHIKVIKTRK NKKDILNPDS SMETSPDFFF
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
Cellular localization
Nuclear
Alternative names
- Gamma-interferon-inducible protein Ifi16 antibody
- Interferon-inducible myeloid differentiation transcriptional activator antibody
Database links
- Entrez Gene: 3428 Human
- Omim: 147586 Human
- SwissProt: Q16666 Human
- Unigene: 380250 Human
Anti-IFI16 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IFI16 antibody (ab55328)
IFI16 antibody (ab55328) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
Western blot - IFI16 antibody (ab55328)
IFI16 antibody (ab55328) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-IFI16 antibody (ab55328)
ab55328 has not yet been referenced specifically in any publications.
Publishing research using ab55328? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"