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Western blot - IFI30 antibody (ab70510)
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Western blot - IFI30 antibody (ab70510)
(enlarge)
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Product Name
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IFI30 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to IFI30
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Immunogen
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Full length protein, corresponding to amino acids 1-250 of Human IFI30
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human spleen lysate and transfected 293T lysate
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Cellular localization
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Lysosome
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Immunology >> Adaptive Immunity >> MHC >> Class II
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Relevance
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The protein encoded by the IFI30 gene is a lysosomal thiol reductase which at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen presenting cells and induced by gamma interferon in other cell types. This enzyme has an important role in MHC class II restricted antigen processing.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IFI30 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IFI30 antibody images, references, products related to ab70510 and other tools.
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IFI30 antibody images:
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 Western blot - IFI30 antibody (ab70510)
IFI30 antibody (ab70510) at 1/500 dilution + Human spleen tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
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Western blot - IFI30 antibody (ab70510)
All lanes : IFI30 antibody (ab70510) at 1/500 dilution
Lane 1 : IFI30 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to IFI30
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IFI30 antibody - more information
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Customer reviews (feedback) regarding IFI30 antibody |
Customer FAQs regarding IFI30 antibody |
Protocols for IFI30 antibody |
Price and availability of products related to IFI30 antibody |
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IFI30 antibody - related products:
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IFI30 antibody - other tools:
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Contact Abcam with a Technical Enquiry about IFI30 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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