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Products:Immunology >> Adaptive Immunity >> MHC >> Class II
Overview
Product name
Anti- IFI30 antibody
See all IFI30 products (3) ...
Description
Mouse polyclonal to IFI30
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-250 of Human IFI30
Positive control
Human spleen lysate and transfected 293T lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Immunology >> Adaptive Immunity >> MHC >> Class II
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The protein encoded by the IFI30 gene is a lysosomal thiol reductase which at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen presenting cells and induced by gamma interferon in other cell types. This enzyme has an important role in MHC class II restricted antigen processing.
Cellular localization
Lysosome
Alternative names
- Gamma interferon inducible lysosomal thiol reductase antibody
- Gamma interferon inducible protein IP 30 antibody
- GILT antibody
see all
Database links
- Entrez Gene: 10437 Human
- Omim: 604664 Human
- SwissProt: P13284 Human
Anti- IFI30 antibody images:
Western blot - IFI30 antibody (ab70510)
Anti- IFI30 antibody (ab70510) at 1/500 dilution + Human spleen tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Western blot - IFI30 antibody (ab70510)
All lanes : Anti- IFI30 antibody (ab70510) at 1/500 dilution
Lane 1 : IFI30 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti- IFI30 antibody (ab70510)
ab70510 has not yet been referenced specifically in any publications.
Publishing research using ab70510? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"