|
|
|
|
Immunocytochemistry/ Immunofluorescence - IFIT2 antibody (ab55837)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - IFIT2 antibody (ab55837)
(enlarge)
|
|
|
|
|
Product Name
|
IFIT2 antibody
|
| |
See all IFIT2 antibodies (2)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to IFIT2
|
|
Immunogen
|
Recombinant full length protein (Mouse)
|
|
Reacts with
|
Ms
|
|
Predicted to react
(species key)
|
Hu
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 25 - 50 µg/ml.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
NIH3T3 cells.
|
|
Cellular localization
|
Intracellular
|
|
Research areas
|
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Immunology >> Innate Immunity >> Macrophage / Inflamm.
|
|
Relevance
|
IFIT2 is a member of the IFIT family family of proteins and contains 6 TPR repeats. It is induced by interferons and is strongly upregulated in peripheral blood in children during the acute phase of influenza.
In mice, IFIT2 protein has been shown to interact with microtubules in vitro, co-localizes with beta-tubulin in vivo and is enriched in the mitotic spindle of non-neuronal cells undergoing mitosis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: None
Constituents: PBS
|
|
Form
|
Liquid
|
|
Concentration
|
2.270 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IFIT2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for IFIT2 antibody images, references, products related to ab55837 and other tools.
|
| |
IFIT2 antibody images:
|
 Immunocytochemistry/ Immunofluorescence - IFIT2 antibody (ab55837)
Ab55837 at 25 µg/ml staining the mitotic spindle in NIH3T3 cells; visualised with TRITC conjugated goat anti rabbit IgG.
|
| |
Immunocytochemistry/ Immunofluorescence - IFIT2 antibody (ab55837)
The top image shows the mitotic spindle of NIH3T3 cells stained by DAPI (4',6-diamidino-2-phenylindole) which fluoresces blue to visualize nuclear DNA.
The middle image shows the mitotic spindle of NIH3T3 cells stained by ab55837 at 50µg/ml using Goat anti-Rabbit IgG conjugated to TRITC (tetramethylrhodamine isothiocyanate) as a secondary antibody.
The bottom image shows the two images above, superimposed on top of each other.
|
| |
References for IFIT2 antibody (ab55837)
|
|
This product has been used in: (two most recent references)
Saha S et al. Characterization of mouse neuronal Ca2+/calmodulin kinase II inhibitor alpha. Brain Res 1148:38-42 (2007). PubMed: 17350603
Saha S et al. Identification of Japanese encephalitis virus-inducible genes in mouse brain and characterization of GARG39/IFIT2 as a microtubule-associated protein. J Gen Virol 87:3285-9 (2006). PubMed: 17030862
If you publish research using ab55837 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to IFIT2
|
| |
IFIT2 antibody - more information
|
Customer reviews (feedback) regarding IFIT2 antibody |
Customer FAQs regarding IFIT2 antibody |
Protocols for IFIT2 antibody |
Price and availability of products related to IFIT2 antibody |
| |
IFIT2 antibody - related products:
|
|
|
| |
IFIT2 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about IFIT2 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
