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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-IFIT2 antibody
See all IFIT2 products (5) ...
Description
Rabbit polyclonal to IFIT2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
Recombinant full length protein (Mouse)
Positive control
NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF: Use a concentration of 25 - 50 µg/ml.
Target
Sequence similarities
Belongs to the IFIT family.
Contains 6 TPR repeats.
Target information above from: UniProt accessionP09913
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cig42 antibody
- G10P2 antibody
- GARG 39 antibody
see all
Database links
- Entrez Gene: 3433 Human
- Entrez Gene: 15958 Mouse
- Omim: 147040 Human
- SwissProt: P09913 Human
- SwissProt: Q64112 Mouse
- Unigene: 437609 Human
- Unigene: 2036 Mouse
Anti-IFIT2 antibody images:
Immunocytochemistry/ Immunofluorescence - IFIT2 antibody (ab55837)
Ab55837 at 25 µg/ml staining the mitotic spindle in NIH3T3 cells; visualised with TRITC conjugated goat anti rabbit IgG.
Immunocytochemistry/ Immunofluorescence - IFIT2 antibody (ab55837)
The top image shows the mitotic spindle of NIH3T3 cells stained by DAPI (4',6-diamidino-2-phenylindole) which fluoresces blue to visualize nuclear DNA. The middle image shows the mitotic spindle of NIH3T3 cells stained by ab55837 at 50µg/ml using Goat anti-Rabbit IgG conjugated to TRITC (tetramethylrhodamine isothiocyanate) as a secondary antibody. The bottom image shows the two images above, superimposed on top of each other.
Western blot - IFIT2 antibody (ab55837)
All lanes : Anti-IFIT2 antibody (ab55837) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A20 (Mouse B lymphoma cell line) Whole Cell Lysate (ab7180)
Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 96 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
References for Anti-IFIT2 antibody (ab55837)
This product has been referenced in:
- Leang RSet al. The Anti-interferon Activity of Conserved Viral dUTPase ORF54 is Essential for an Effective MHV-68 Infection. PLoS Pathog 7:e1002292 (2011). WB; Mouse.Read more (PubMed: 21998588) »
See 1 publication for this product
Publishing research using ab55837? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"