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IFN gamma Receptor beta protein (Fc Chimera) (ab83988)

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Overview

  • Product nameIFN gamma Receptor beta protein (Fc Chimera)See all IFN gamma Receptor beta proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to the extracellular domain (amino acids 28-247) of Human IFN gamma Receptor beta (Swiss-Prot P38484), fused to the Fc region of Human IgG1 (aa 93-330). The chimeric protein was expressed in modified Human 293 cells.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical sequence: SQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYRV QFKYTDSKWF TADIMSIGVNCTQITATECDFTAASPSA GFPMDFNVTLRLRAELGALH SAWVTMPWFQHYRNVTVG PPENIEVTPGEGSLIIRFSSPFDIADTSTA FFCYYVHY WEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWN KSNIFRVGHLSNISCYETMADASTELQQGSSNTKVDKK VEPKSCDKTH TCPPCPAPELLGGPSVFLFPPKPKDTLM ISRTPEVTCVVVDVSHEDPE VKFNWYVDGVEVHNAKTK PREEQYNSTYRVVSVLTVLHQDWLNGKEYK CKVSNKAL PAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL YSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLS PGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83988 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    ab83988 migrates as a broad band between 65 and 75 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IFN gamma Receptor beta (Fc Chimera) that has a predicted molecular mass of 52 kDa. ab83988 consists of 20-30% carbohydrate by weight.

    ab83988 separates into a number of glycoforms with a pI between 5.5 and 8 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IFN gamma Receptor beta (Fc Chimera) that has a predicted pI of 7.5.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AF 1AF-1AF1
        IFGR 2IFGR2IFN-gamma receptor 2IFN-gamma-R2IFNGR 2IFNGR2IFNGT 1IFNGT1INGR2_HUMANInterferon gamma receptor 2Interferon gamma receptor accessory factor 1Interferon gamma receptor beta chainInterferon gamma receptor chain 2Interferon gamma transducer 1
      see all
  • FunctionPart of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
  • Involvement in diseaseDefects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similaritiesBelongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P38484 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IFN gamma Receptor beta protein (Fc Chimera) images

    • SDS-PAGE - IFN gamma Receptor beta protein (Fc Chimera) (ab83988)
      SDS-PAGE - IFN gamma Receptor beta protein (Fc Chimera) (ab83988)
      Lane 1 – ab83988; Lane 2 – ab83988 treated with PNGase F to remove potential N-linked glycans; Lane 3 – ab83988 treated with a glycosidase cocktail to remove potential N- and O-linked glycans; Lane 4 – MW markers. 10 µg of protein was loaded per lane; Gel was stained with Coomassie G250.

      Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
    • Functional Studies - IFN gamma Receptor beta protein (Fc Chimera) (ab83988)
      Functional Studies - IFN gamma Receptor beta protein (Fc Chimera) (ab83988)
      Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.

      The triangle indicates theoretical pI and MW of the protein.

    References for IFN gamma Receptor beta protein (Fc Chimera) (ab83988)

    ab83988 has not yet been referenced specifically in any publications.

    Publishing research using ab83988 ? Please let us know so that we can cite the reference in this datasheet

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