Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Innate Immunity >> Cytokines >> Interferons
Overview
Product name
Anti-IFNA4 antibody
Description
Mouse polyclonal to IFNA4
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-189 of Human IFNA4
Positive control
Transfected 293Tcell lysate (WB) HeLa cells (ICC/IF)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor immunology >> Cytokines >> Interferons
Immunology >> Innate Immunity >> Cytokines >> Interferons
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 10 µg/ml. Purification with Protein A is recommended prior to proceeding with the ICC/IF protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Produced by macrophages, IFN alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Cellular localization
Secreted
Alternative names
- Interferon alpha 4 precursor antibody
- Interferon alpha 4B antibody
- Interferon alpha 76 antibody
see all
Database links
- Entrez Gene: 3441 Human
- Omim: 147564 Human
- SwissProt: P05014 Human
- Unigene: 1510 Human
Anti-IFNA4 antibody images:
Immunocytochemistry/ Immunofluorescence - IFNA4 antibody (ab67385)
ab67385, at a concentration 10 µg/ml, staining IFNA4 in HeLa cell by Immunofluorescence.
Western blot - IFNA4 antibody (ab67385)
All lanes : Anti-IFNA4 antibody (ab67385) at 1/500 dilution
Lane 1 : IFNA4 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 22 kDa
Observed band size : 18,20 kDa (why is the actual band size different from the predicted?)
References for Anti-IFNA4 antibody (ab67385)
ab67385 has not yet been referenced specifically in any publications.
Publishing research using ab67385? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"