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Immunocytochemistry/ Immunofluorescence-IFNAR2 antibody(ab56070)
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Product Name
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IFNAR2 antibody
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See all IFNAR2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to IFNAR2
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Immunogen
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A synthetic peptide corresponding to C terminal residues of human IFNAR2 (Interferon-alpha/beta receptor beta chain precursor)
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Reacts with
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Hu
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1.0 µg/ml for 2hrs. Predicted molecular weight: 58 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; single pass type I membrane protein
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Research areas
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Cancer >> Tumor immunology >> Cytokines >> Interferons
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Immunology >> Innate Immunity >> Cytokines >> Interferons
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Relevance
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IFNAR2 is a receptor for interferons alpha and beta. Isoform 1 and isoform 3 are directly involved in signal transduction due to their interaction with the TYR kinase, JAK1. Isoform 1 also interacts with the transcriptional factors, STAT1 and STAT2. Both forms are potent inhibitors of type I IFN activity
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IFNAR2 antibody (ab56070) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified by antigen specific affinity chromatography.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IFNAR2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IFNAR2 antibody images, references, products related to ab56070 and other tools.
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IFNAR2 antibody images:
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 Immunocytochemistry/ Immunofluorescence-IFNAR2 antibody(ab56070)
ICC/IF image of ab56070 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56070, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for IFNAR2 antibody (ab56070)
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This product has been used in:
Ghosh D et al. A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress. Proc Natl Acad Sci U S A 105:11673-8 (2008). IP; Human. PubMed: 18687895
If you publish research using ab56070 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IFNAR2
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IFNAR2 antibody - more information
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Customer reviews (feedback) regarding IFNAR2 antibody |
Customer FAQs regarding IFNAR2 antibody |
Protocols for IFNAR2 antibody |
Price and availability of products related to IFNAR2 antibody |
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IFNAR2 antibody - related products:
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IFNAR2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about IFNAR2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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