You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-IFNAR2 antibody (ab56070)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-IFNAR2 antibody
See all IFNAR2 products (4) ...

Description

Rabbit polyclonal to IFNAR2

Tested applications

ICC/IF, WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

A synthetic peptide corresponding to C terminal residues of human IFNAR2 (Interferon-alpha/beta receptor beta chain precursor)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified by antigen specific affinity chromatography.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Tumor immunology >> Cytokines >> Interferons
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Immunology >> Innate Immunity >> Cytokines >> Interferons

  • Immunocytochemistry/ Immunofluorescence-IFNAR2 antibody(ab56070)Immunocytochemistry/ Immunofluorescence-IFNAR2 antibody(ab56070) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab56070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    ICC/IF

    Four stars (1 Abreview) ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 µg/ml

  • ShowHide

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1.0 µg/mlPredicted molecular weight: 58 kDa.(Two hour incubation. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)

  • ShowHide

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

Target

Function

Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity.

Tissue specificity

Isoform 3 is detected in the urine (at protein level). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines.

Sequence similarities

Belongs to the type II cytokine receptor family.

Post-translational
modifications

Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.
Glycosylated.

Cellular localization

Secreted and Membrane.

Target information above from: UniProt accessionP48551 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • IFN alpha REC antibody
  • IFN-alpha binding protein antibody
  • IFN-alpha/beta receptor 2 antibody
  • IFN-R-2 antibody
  • IFNABR antibody
  • IFNAR2 antibody
  • IFNARB antibody
  • IFNR antibody
  • INAR2_HUMAN antibody
  • Interferon alpha binding protein antibody
  • Interferon alpha/beta receptor 2 antibody
  • Interferon alpha/beta receptor beta chain antibody
  • Type I interferon receptor 2 antibody
  • Type I interferon receptor antibody
see all

Database links

Anti-IFNAR2 antibody images:

  Immunocytochemistry/ Immunofluorescence-IFNAR2 antibody(ab56070)

Immunocytochemistry/ Immunofluorescence-IFNAR2 antibody(ab56070)

ICC/IF image of ab56070 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56070, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-IFNAR2 antibody (ab56070)

This product has been referenced in:

  • Ghosh Det al. A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress. Proc Natl Acad Sci U S A 105:11673-8 (2008). IP; Human.Read more (PubMed: 18687895) »

See 1 publication for this product

Publishing research using ab56070? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"