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Overview

  • Product nameIFNAR2 protein (Active)See all IFNAR2 proteins and peptides ...
  • Protein descriptionRecombinant Human IFNAR2 (aa 27-243 extracellular domain) fused to the Fc region of human IgG1 (aa 90-330), expressed in modified human 293 cells, predicted 52kDa but migrates as a broad band between 60 and 85 kDa. Swiss ID = P48551.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityab83680 bound to protein A sepharose beads was able to pull down its ligand, IFNa2b.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Additional notesab83680 bound to protein A sepharose beads was able to pull down its ligand, IFNa2b.
  • Sequence notesTheoretical Sequence: ISYDSPDYTD ESCTFKISLR NFRSILSWEL KNHSIVPTHY TLLYTIMSKP EDLKVVKNCA NTTRSFCDLT DEWRSTHEAY VTVLEGFSGN TTLFSCSHNF WLAIDMSFEP PEFEIVGFTN HINVMVKFPS IVEEELQFDL SLVIEEQSEG IVKKHKPEIK GNMSGNFTYI IDKLIPNTNY CVSVYLEHSD EQAVIKSPLK CTLLPPGQES ESAESAKGSS NTKVDKKVEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83680 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab104451.

    • Protein info

    • Alternative names
        Human interferon alpha/beta receptorIFN alpha RECIFN R
        IFN-alpha binding proteinIFN-alpha/beta receptor 2IFN-R-2IFNABRIFNAR2IFNARBIFNRINAR2_HUMANInterferon (alpha beta and omega) receptor 2Interferon alpha binding proteinInterferon alpha/beta receptor 2Interferon alpha/beta receptor beta chainType I interferon receptorType I interferon receptor 2
      see all
  • FunctionAssociates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity.
  • Tissue specificityIsoform 3 is detected in the urine (at protein level). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines.
  • Sequence similaritiesBelongs to the type II cytokine receptor family.
  • Post-translational
    modifications    Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.
    Glycosylated.
  • Cellular localizationSecreted and Membrane.

    • Target information above from: UniProt accession P48551 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IFNAR2 protein (Active) images

    • Functional Studies - IFNAR2 protein (Active) (ab83680)
      Functional Studies - IFNAR2 protein (Active) (ab83680)
      Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - IFNAR2 protein (Fc Chimera Active) (ab83680)
      SDS-PAGE - IFNAR2 protein (Fc Chimera Active) (ab83680)
      1D SDS-PAGE of ab83680 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1 MW markers; Lane 2 ab83680; Lane 3 ab83680 treated with PNGase F to remove potential N-linked glycans; Lane 4 ab83680 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

    • SDS-PAGE - IFNAR2 protein (Active) (ab83680)
      SDS-PAGE - IFNAR2 protein (Active) (ab83680)
      A sample of ab83680 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 µg of protein was load; Gel was stained using Deep Purple™.

    References for IFNAR2 protein (Active) (ab83680)

    ab83680 has not yet been referenced specifically in any publications.

    Publishing research using ab83680 ? Please let us know so that we can cite the reference in this datasheet

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