IFNAR2 protein (Active) (ab83680)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab83680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- Human interferon alpha/beta receptorIFN alpha RECIFN R
- IFN-alpha binding proteinIFN-alpha/beta receptor 2IFN-R-2IFNABRIFNAR2IFNARBIFNRINAR2_HUMANInterferon (alpha beta and omega) receptor 2Interferon alpha binding proteinInterferon alpha/beta receptor 2Interferon alpha/beta receptor beta chainType I interferon receptorType I interferon receptor 2
modificationsPhosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.
IFNAR2 protein (Active) images
Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83680 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 MW markers; Lane 2 ab83680; Lane 3 ab83680 treated with PNGase F to remove potential N-linked glycans; Lane 4 ab83680 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
A sample of ab83680 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 µg of protein was load; Gel was stained using Deep Purple™.
References for IFNAR2 protein (Active) (ab83680)
ab83680 has not yet been referenced specifically in any publications.