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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Anti-IGF1 Receptor antibody [alphaIR3] (ab16890)
Product partner
Related products
- Secondary antibodies
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab16890? Please let us know so that we can cite the reference in this datasheet
ab16890 has been referenced in 3 publications.
- Jaquish DV et al. IGF1-R signals through the RON receptor to mediate pancreatic cancer cell migration. Carcinogenesis 32:1151-6 (2011). ICC/IF; Human. PubMed: 21565828
- Rohlik QT et al. An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture. Biochem Biophys Res Commun 149:276-81 (1987). PubMed: 2961338
- Kull FC et al. Monoclonal antibodies to receptors for insulin and somatomedin-C. J Biol Chem 258:6561-6 (1983). PubMed: 6304046
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - Anti-IGF1 Receptor antibody [alphaIR3] (ab16890)](/ps/datasheet/images/16/ab16890/IGF1-Receptor-Primary-antibodies-ab16890-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IGF1 Receptor antibody [alphaIR3] (ab16890)
ICC/IF image of ab16890 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16890, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry - IGF1 Receptor antibody [alphaIR3] (ab16890)](/ps/datasheet/images/16/ab16890/IGF1-Receptor-Primary-antibodies-ab16890-2.jpg)
Flow Cytometry - IGF1 Receptor antibody [alphaIR3] (ab16890)
Overlay histogram showing LoVo cells stained with ab16890 (red line). The cells were fixed with 4% paraformaldehyde (10 mins) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16890, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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