Anti-IGF1 Receptor antibody [alphaIR3] (ab16890)

Ich bräuchte für eine Versuchsreihe an Prostatakarzinom Zellinieneinen blockierenden Antikörper gegen den IGF- 1 Rezeptor. Da es bei Ihnen einige gibt, wollte ich Sie frage, ob Sie mir einen empfehlen könnten? Für mich ist es wichtig, dass der Antikörper eine blockierende Wirkung hat sowie Azide-free ist.

ANSWER:

Vielen Dank für Ihre Anfrage.

Der ab16890 Anti-IGF1 Receptor antibody [alphaIR3] sollte gut für Ihre Zwecke geeignet sein:

Er wurde bereits im Funktional Assay getestet
Er blockiert den Rezeptor (allerdings auch zu einem gewißen Grad den Insulin Rezeptor)
Der Puffer besteht nur aus PBS

Does this antibody react to the alpha or beta subunit of this receptor?

ANSWER:

Thank you for your enquiry. This antibody recognizes the ~130 kDa a (alpha) and the ~90 kDa b (beta) subunits of IGF-I receptor.

Please do not hesitate to contact us for further assistance.

Product "reconstituted in phosphate buffered saline (PBS), pH 7.4, or sterile 20mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml"

ANSWER:

Thank you for your enquiry.

Further to correspondence with my colleagues in the lab I can tell you that ab16890 is in fact reconstituted in phosphate buffered saline (PBS), pH 7.4. I have updated the datasheet to reflect this.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Product "reconstituted in phosphate buffered saline (PBS), pH 7.4, or sterile 20mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml"

ANSWER:

Thank you for your enquiry.

Further to correspondence with my colleagues in the lab I can tell you that ab16890 is in fact reconstituted in phosphate buffered saline (PBS), pH 7.4.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

In our immunohistochemistry lab, we are currently working on IGF1R. After performing work on human tissue, we would like to pursue on rat tissue.

We used your monoclonal antibody ab4065, reacting well with human tissue. A test in rat confirmed that it does not react.

Now we would like to pursue on rat tissue. We saw that your monoclonal antibodies to IGF1R : ab16890, ab16817, ab15321 were not tested in rat (according to the website). Is it possible for you to send us test samples of those three antisera. We would like to test them in rat. If positive, we would, of course, order more. Additionally, we would send you documentation about positive/negative results, you could add to the website, as our lab of immunohistochemistry, Leuven, KUL university hospital has a lot of experience.

Many thanks for your attention and for an answer in the next few days,

ANSWER:

Thank you for your enquiry.

Free or trial sized samples are considered on a case by case basis. On this occasion unfortunately I cannot offer you a free sample. However, should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image).

Please can I encourage you to leave your valuable contribution of the performance of ab4065 in rat by searching for the antibody at abcam.com and clicking on the "abreview" tab. In return we will offer you 50 Abcam Points.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.