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Immunocytochemistry/ Immunofluorescence-IGF1 Receptor antibody [alphaIR3](ab16890)
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Product Name
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IGF1 Receptor antibody [alphaIR3]
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See all IGF1 Receptor antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [alphaIR3] to IGF1 Receptor
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Immunogen
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Immunogen used was partially purified receptor from human placenta.
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Reacts with
(species key)
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Hu, Hm
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Specificity
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Recognizes the human IGF-I Receptor. IGF-I Receptor (Ab-1)immunoprecipitates the a and ß subunits of the IGF-I receptor. IGF-I receptor (ab16890) blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). This antibody is not recommended for Western blotting or paraffin sections.
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Tested applications
(see key)
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ICC, IP, Neut
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC (IF): Use at a concentration of 1 - 5 µg/ml.
IP: Use at an assay dependent dilution.
Neut: Use at a concentration of 1 µg/ml.
Is unsuitable for IHC-P or WB.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cells
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Cellular localization
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Type I membrane protein
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Research areas
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Developmental Biology >> Post embryonic development >> Aging
Cancer >> Growth factors >> Insulin and insulin-like
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Relevance
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There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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alphaIR3
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.4
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IGF1 Receptor antibody [alphaIR3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IGF1 Receptor antibody [alphaIR3] images, references, products related to ab16890 and other tools.
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IGF1 Receptor antibody [alphaIR3] images:
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 Immunocytochemistry/ Immunofluorescence-IGF1 Receptor antibody [alphaIR3](ab16890)
ICC/IF image of ab16890 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16890, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for IGF1 Receptor antibody [alphaIR3] (ab16890)
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This product has been used in: (two most recent references)
Rohlik QT et al. An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture. Biochem Biophys Res Commun 149:276-81 (1987). PubMed: 2961338
Kull FC et al. Monoclonal antibodies to receptors for insulin and somatomedin-C. J Biol Chem 258:6561-6 (1983). PubMed: 6304046
If you publish research using ab16890 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IGF1 Receptor
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IGF1 Receptor antibody [alphaIR3] - more information
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IGF1 Receptor antibody [alphaIR3] - related products:
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IGF1 Receptor antibody [alphaIR3] - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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