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IGF1 Receptor beta subunit protein (Active) (ab80349)

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Overview

  • Product nameIGF1 Receptor beta subunit protein (Active)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 930-1337 of the cytoplasmic domain of Human IGF1 Receptor beta subunit fused with a proprietary tag; expressed in a baculovirus infected Sf9 cells, 72.3 kDa. Swiss ID = P08069.
  • Expression hostInsect cells

    • Properties

  • Purification notesPurity is >80% by SDS-PAGE.
  • Biological activitySpecific activity: ~100 nmol/min/mg at 25°C using poly(Glu:Tyr)4:1 substrate. Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 1 mM DTT, 250 µM ATP. The kinase concentration was 0.5-1 µM and poly-L-lysine was added in equimolar amounts to the kinase.
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 50mM Tris, 1mM DTT, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab80349 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. May also be useful for enzyme kinetics, autophosphorylation and phosphorylation of target substrates.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        CD221CD221 antigenEC 2.7.10.1
        IGF I receptorIGF-I receptorIGF1RIGF1R_HUMANIGFIRInsulin like growth factor 1 receptor alpha chainInsulin like growth factor 1 receptor beta chainInsulin like growth factor I receptorInsulin-like growth factor 1 receptor beta chainInsulin-like growth factor I receptorJTK13MGC142170MGC142172MGC18216Soluble IGF1R variant 1Soluble IGF1R variant 2
      see all
  • FunctionThis receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
  • Tissue specificityFound as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
  • Involvement in diseaseDefects in IGF1R are a cause of insulin-like growth factor 1 resistance (IGF1RES) [MIM:270450]. It is a disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 3 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
    Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P08069 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for IGF1 Receptor beta subunit protein (Active) (ab80349)

    ab80349 has not yet been referenced specifically in any publications.

    Publishing research using ab80349 ? Please let us know so that we can cite the reference in this datasheet

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    Inquiry: I am looking for a phospho-IGF1R recombinant protein standard for ELISAs. The more of the native protein present, the better. Does this product number contain p-IGF1R beta subunits or would I need to phosphorylate it in vitro? thanks!

    Thank you for contacting us.

    This productdoesn’t contain p-IGF-1R beta and doesn’t need to phosphorylate it in vitro for the enzyme activity.


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