IGF1 Receptor (phospho Y1161) antibody (ab39398)

Product Name 

IGF1 Receptor (phospho Y1161) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to IGF1 Receptor (phospho Y1161)

Immunogen 

Synthetic phospho-peptide derived from human IGF1 receptor around the phosphorylation site of tyrosine 1161

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Ab39398 detects endogenous levels of IGF1 Receptor only when phosphorylated at tyrosine 1161. The antibody cross-reacts with insulin receptor at phosphorylation site Y1185.

Tested applications 
(see key)

ELISA, IHC-FoFr, IHC-P, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
IHC-FoFr: 1/100 (PMID 18216249).
IP: Use at an assay dependent dilution.
WB: 1/50 - 1/100. A 180kD band for the precursor (predicted at 155kD) and a 95kD for the beta chain is often observed in reduced conditions.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein

Research areas 

Developmental Biology >> Post embryonic development >> Aging
Cancer >> Growth factors >> Insulin and insulin-like
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like

Relevance 

There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg⁺⁺ and Ca⁺⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab39398 was affinity purified from rabbit antiserum by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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References for IGF1 Receptor (phospho Y1161) antibody (ab39398)

  This product has been used in: (two most recent references)

Bitoun E et al. AF4 is a critical regulator of the IGF-1 signaling pathway during Purkinje cell development. J Neurosci 29:15366-74 (2009). IHC-P; Mouse. PubMed: 20007461

Gatchel JR et al. The insulin-like growth factor pathway is altered in spinocerebellar ataxia type 1 and type 7. Proc Natl Acad Sci U S A 105:1291-6 (2008). WB, IHC-FoFr; Mouse. PubMed: 18216249
If you publish research using ab39398 please let us know so that we can cite the reference on this datasheet.

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