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Western blot - IGF1 EA antibody (ab22187)
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Product Name
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IGF1 antibody
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See all IGF1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to IGF1
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Immunogen
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Fusion protein: EVHLKNTSRGSAGNKTYRM, corresponding to amino acids 135/153 of Mouse IGF1 EA
BLAST 'EVHLKNTSRGSAGNKTYRM' with  or 
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Reacts with
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Ms
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Predicted to react
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Rat
(Due to sequence homology)
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 17 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Secreted
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Research areas
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Developmental Biology >> Post embryonic development >> Growth hormones
Developmental Biology >> Post embryonic development >> Aging
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Relevance
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Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IGF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IGF1 antibody images, references, products related to ab22187 and other tools.
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IGF1 antibody images:
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 Western blot - IGF1 EA antibody (ab22187)
All lanes : IGF1 antibody (ab22187) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 17 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to IGF1
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IGF1 antibody - more information
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Customer reviews (feedback) regarding IGF1 antibody |
Customer FAQs regarding IGF1 antibody |
Protocols for IGF1 antibody |
Price and availability of products related to IGF1 antibody |
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IGF1 antibody - related products:
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IGF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about IGF1 antibody |
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