Overview

  • Product nameAnti-IGF1 antibodySee all IGF1 primary antibodies ...
  • Description
    Mouse monoclonal to IGF1
  • SpecificityAs determined by EIA, this antibody shows 100 % reactivity with rat and mouse IGF1. It also shows <0.4 % cross reactivity with rat IGF2, and <0.05 % cross reactivity with human IGF1 or IGF2.
  • Tested applicationsDot Blot, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat

    Does not react with

    Human
  • Immunogen

    Recombinant full length protein (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab36532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot 1/5000.
WB 1/5000. Predicted molecular weight: 7.5 kDa.

Detects 250 ng IGF1. The predicted MW corresponds to the mature form of the protein.

ELISA 1/2000.

Target

  • FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P05019 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names
    • IBP1 antibody
    • IGF 1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin Like Growth Factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Anti-IGF1 antibody images

  • All lanes : Anti-IGF1 antibody (ab36532) at 1/2500 dilution

    Lane 1 : Rat IGF1 at 1.5 µg
    Lane 2 : Rat IGF1 at 0.75 µg
    Lane 3 : Rat IGF1 at 0.375 µg
    Lane 4 : Rat IGF1 at 0.1875 µg
    Lane 5 : Rat IGF1 at 0.09375 µg

    Secondary
    HRP-conjugated anti-mouse IgG at 1/2000 dilution
    developed using the ECL technique

    Predicted band size : 7.5 kDa

    Monomer, dimer and trimer bands present.

References for Anti-IGF1 antibody (ab36532)

This product has been referenced in:
  • Fernández S  et al. A phosphatase-independent gain-of-function mutation in PTEN triggers aberrant cell growth in astrocytes through an autocrine IGF-1 loop. Oncogene N/A:N/A (2013). Read more (PubMed: 24056966) »
  • Hamzeh M & Robaire B Identification of early response genes and pathway activated by androgens in the initial segment and caput regions of the regressed rat epididymis. Endocrinology 151:4504-14 (2010). WB ; Rat . Read more (PubMed: 20660069) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

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Submitted Jun 30 2012

Thank you for contacting us and for participating in our testing program.

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 21 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"