Anti-IGF1 antibody (ab40657)

I just submitted an abreview of the tested antibody but forgot to input the discount code on the "additional comments" box. Could you please activate the discount (********) once the review is accepted?
Thanks so much.

ANSWER:

Thank you for contacting us and for your Abreview.

I have linked the discount code to your Abreview. The code is now activated, you can place an order for a free antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hello,
I would like to test the Anti-IGF1 antibody (ab40657) in IHC ofparaffin-embedded mouse prostate tissue. According to the datasheet, this antibody recognizes human IGF1, but it hasn't been tested on mouse.
Could you let me know if I would get the 100% testing discount if I do the test and submit the abreview?
Also, I welcome any suggestions for the test that might not be included in the datasheet. Or suggestions of other antibodies that might be more suitable for my needs.
Thanks for your help.
Kind regards,

ANSWER:

DISCOUNT CODE: ******
Expiration date: JJ MM YYYY

I am very pleased to hear you would like to accept our offer and test ab40657 in Mouse (100% homology with the immunogen). This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forMouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Dear technical team,   Our customer would like to purchase ab40657 and has inquiry about immnogen information. http://www.abcam.com/IGF1-antibody-ab40657.html   It described as "Synthetic human insulin-like growth factor 1 [Ac60-70] covalently attached onto a carrier protein."   1. Why is this attached onto a carrier protein?   The customer asked about the role of carrier protein and effect on antibody activity.   2.  What is the meaning of "Ac" ([Ac60-70] )?   Check them please.   Thank you.    

ANSWER:

Thank you for your enquiry and your interest in our products.

Native human IGF-1 is a 70 amino acid long peptide. We wanted to develop an antibody that bounds specifically to the -COOH terminus of the whole peptide, so we synthesized an 11-mer, i.e. residues #60-70 aa - to use as immunogen.

Since we didn't want a free NH2-group at the amino terminal, we capped the amino terminus during synthesis by acetylating it with acetic anhydride which adds an acetyl group, i.e. the Ac moiety. Thus the resulting synthetic analogue of the carboxyl terminal region is Ac60-70.

This synthetic peptide was coupled onto bovine thyroglobulin as carrier protein to elicit an antibody response since the immune system will not recognize small peptides, such as this 11-amino acid long analogue, as an antigen without it being conjugated onto a much larger protein.

I hope this helps and if I can assist further, please do not hesitate to contact me.

We received a lyophilised antibody. data sheet said dilute with 0.1 ml 0f 10mg/mlBSA in PBS. Please simplify. we have only PBS of distilled water as diluent. how many ml do we have to add.please answer asap. thanks.

ANSWER:

Thank you for your enquiry.

I am sorry to hear that you are having problems with using one of our products.

This product is supplied as lyophilized powder, which should be stored at 4oC until rehydrated. Histochemical grade antibodies should be diluted with PBS that contains either 1.0% BSA or 1% normal goat serum, aliquoted into single use portions, and stored frozen at -20C: do not repeatedly freeze and thaw.

I hope this information will be useful. Please do not hesitate to contact me again if you require further information.

Good luck in your research.