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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-IGF1 antibody
See all IGF1 products (13) ...
Description
Rabbit polyclonal to IGF1
Tested applications
IHC-P, WB, ELISA, Neutralisingmore details
Cross reactivity
Reacts with
Human
Immunogen
Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IGF1 antibody (ab9572)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent concentration. (To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.)
ELISA: Use at an assay dependent concentration. (To detect hIGF-1 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-1.)
Neut: Use at an assay dependent concentration. (To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.)
Target
Relevance
Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.
Cellular localization
Secreted
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
Alternative names
- IBP1 antibody
- IGF 1 antibody
- IGF I antibody
see all
Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
Anti-IGF1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IGF1 antibody (ab9572)
Ab9572 staining Human normal liver parenchyma. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-IGF1 antibody (ab9572)
This product has been referenced in:
- Sprynski ACet al. The role of IGF-1 as a major growth factor for myeloma cell lines and the prognostic relevance of the expression of its receptor. Blood 113:4614-26 (2009). WB; Human.Read more (PubMed: 19228610) »
- Bitoun Eet al. AF4 is a critical regulator of the IGF-1 signaling pathway during Purkinje cell development. J Neurosci 29:15366-74 (2009). IHC-P; Mouse.Read more (PubMed: 20007461) »
See all 4 publications for this product
Publishing research using ab9572? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"