Overview
Product nameIGF1 protein (Active)See all IGF1 proteins and peptides ...
Protein descriptionRecombinant full length Gilthead Seabream IGF1; 68 amino acids, 7545.4 Dalton. Swiss ID = Q56V57. IGF1 was prepared according to the sequenced published in Fine M et al. Recombinant gilthead seabream (Sparus aurata) insulin-like growth factor-I: subcloning, expression in Escherichia coli, purification and characterization. J Endocrinol 153:139-50 (1997). PubMed: 9135579
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab73850 is purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
Biological activityBiological Activity:
Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGFI as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF1. Recombinant Gilthead Seabream IGF1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGFI and hIGFI measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGFI is biologically active.
FormLyophilised:Reconstitute in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab73850 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 0.02% NaHCO3
Concentration information loading...
Sequence notesThe sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pro-Glu-Thr-Leu-Cys-Gly-Ala-Glu.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab73850
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| Functional Studies |
FuncS: Use at an assay dependent dilution. |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
IGF IAIGF IBIGF-IIgf1IGF1_HUMANIGFIIGFIAInsulin like growth factor 1Insulin like growth factor 1 (somatomedin C)Insulin like growth factor IAInsulin like growth factor IBInsulin-like growth factor IMechano growth factorMGFOTTHUMP00000195080OTTHUMP00000195081OTTHUMP00000195082OTTHUMP00000195083OTTHUMP00000195084Somatomedia CSomatomedin CSomatomedin-C
see all
FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
Sequence similaritiesBelongs to the insulin family.
Cellular localizationSecreted.
References for IGF1 protein (Active) (ab73850)
ab73850
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
