Overview
- Product nameAnti-IGF2 antibody [RM0100-3J42]See all IGF2 primary antibodies ...
- DescriptionRat monoclonal [RM0100-3J42] to IGF2
- Tested applicationsSandwich ELISA, WB more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Recombinant full length Mouse protein
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... - Purification notesThe IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and 0.2µm filtered.
- Clonality Monoclonal
- Clone numberRM0100-3J42
- IsotypeIgG2
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab86485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF2 (ab18954). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab18954 as Detection. |
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 20 kDa. |
Target
- FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. - Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
- Sequence similaritiesBelongs to the insulin family.
- Post-translational
modificationsO-glycosylated with a core 1 or possibly core 8 glycan. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 3481 Human
- Entrez Gene: 16002 Mouse
- Omim: 147470 Human
- SwissProt: P01344 Human
- SwissProt: P09535 Mouse
- Unigene: 272259 Human
- Unigene: 718427 Human
- Unigene: 3862 Mouse
Target information above from: UniProt accession
P01344
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- C11orf43 antibodyIGF 2 antibodyIGF II antibody
- IGF-II antibodyIGF2 antibodyIGF2_HUMAN antibodyIGFII antibodyINSIGF antibodyinsulin like growth factor 2 (somatomedin A) antibodyInsulin like Growth Factor 2 antibodyInsulin like growth factor II antibodyInsulin like growth factor II precursor antibodyInsulin like growth factor type 2 antibodypp9974 antibodyPreptin antibodyputative insulin like growth factor II associated protein antibodySomatomedin A antibodySomatomedin-A antibody
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Anti-IGF2 antibody [RM0100-3J42] images
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![Sandwich ELISA - IGF2 antibody [RM0100-3J42] (ab86485)](http://a.abcam.com/ps/datasheet/images/86/ab86485/IGF2-Primary-antibodies-ab86485-3.jpg)
Sandwich ELISA - IGF2 antibody [RM0100-3J42] (ab86485)Standard Curve for IGF2 (Analyte: ab9575) dilution range 1pg/ml to 1ug/ml using Capture Antibody Rat monoclonal [RM0100-3J42] to IGF2 (ab86485) at 1ug/ml and Detector Antibody Rabbit polyclonal to IGF2 (ab18954) at 0.5ug/ml.
References for Anti-IGF2 antibody [RM0100-3J42] (ab86485)
ab86485 has not yet been referenced specifically in any publications.
