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Overview

  • Product nameIGF2 protein (Active)See all IGF2 proteins and peptides ...
  • Protein descriptionHuman IGF-2.
  • Expression hostE. coli

    • Properties

  • Purification notesSterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Reconstitute with 100ul of water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesThe ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of >5 x 105 units/mg.
  • Sequence notesHuman IGF-2 is a 7.5 kDa protein containing 67 amino acid residues: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEEC CFRS CDLALLETYCATPAKSE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab9575 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab9574 and ab170304.

    • Protein info

    • Alternative names
        C11orf43FLJ22066FLJ44734
        IGF 2IGF IIIGF-IIIGF2IGF2_HUMANIGFIIINSIGFInsulin like Growth Factor 2insulin like growth factor 2 (somatomedin A)Insulin like growth factor IIInsulin like growth factor II precursorInsulin like growth factor type 2Insulin-like Growth Factor 2Insulin-like Growth Factor 2pp9974Preptinputative insulin like growth factor II associated proteinSomatomedin ASomatomedin ASomatomedin-A
      see all
  • FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similaritiesBelongs to the insulin family.
  • Post-translational
    modifications    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01344 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IGF2 protein (Active) images

    • Sandwich ELISA - IGF2 protein (ab9575)
      Sandwich ELISA - IGF2 protein (ab9575)
      Standard curve for IGF2 (Analyte: ab9575); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab86485 at 1µg/ml and Detector Antibody ab18954 at 0.5µg/ml.

    References for IGF2 protein (Active) (ab9575)

    This product has been referenced in:
    • Suvasini R  et al. Insulin Growth Factor-2 Binding Protein 3 (IGF2BP3) Is a Glioblastoma-specific Marker That Activates Phosphatidylinositol 3-Kinase/Mitogen-activated Protein Kinase (PI3K/MAPK) Pathways by Modulating IGF-2. J Biol Chem 286:25882-90 (2011). Read more (PubMed: 21613208) »

    See 1 Publication for this product

    Publishing research using ab9575 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab9575 Submit a Question for ab9575

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    Thanks for the information! I think we want to get the Igf2 antibody. Do you know if this one has the control peptide?

    We have several IGF2 antibodies but the immunogens for each are not all commercially available, and not all are raised against peptides. For the example I gave, ab9574, the immunogen is full-length recombinant IGF2, and this is available as ab9575:
    ...

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    A few months ago I ordered the IGF2 antibody ab9574. I would like to know if there is a blocking peptide available for this antibody. Could you please send me some information?  

    The blocking protein would be ab9575. Please see datasheet for specifics.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"