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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
Anti-IGFBP1 antibody [33627.11]
See all IGFBP1 products (7) ...
Description
Mouse monoclonal [33627.11] to IGFBP1
Specificity
No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4.
Tested applications
WB, ELISA, Neutralisingmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
33627.11
Isotype
IgG1
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
Neutralising
Application notes
ELISA: Use at a concentration of 2 µg/ml. In the ELISA capture assay, plates are coated with 100 µl/well of the capture antibody (2 µg/ml) in combination with 100 µl/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained.
Neut: Use at a concentration of 10 - 40 µg/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 µg/ml in the presence of approximately 5 µg/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Post-translational
modifications
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
Cellular localization
Secreted.
Target information above from: UniProt accessionP08833
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFBP antibody
- Alpha pregnancy associated endometrial globulin antibody
- Amniotic fluid binding protein antibody
see all
Database links
- Entrez Gene: 3484 Human
- Omim: 146730 Human
- SwissProt: P08833 Human
- SwissProt: Q6PEY6 Human
- Unigene: 401316 Human
- Unigene: 642938 Human
References for Anti-IGFBP1 antibody [33627.11] (ab10732)
This product has been referenced in:
- Walker Get al. Insulin-like growth factor binding proteins IGFBP3, IGFBP4, and IGFBP5 predict endocrine responsiveness in patients with ovarian cancer. Clin Cancer Res 13:1438-44 (2007).Read more (PubMed: 17332286) »
See 1 publication for this product
Publishing research using ab10732? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"