IGFBP1 antibody [33627.11] (ab10732)

Product Name 

IGFBP1 antibody [33627.11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [33627.11] to IGFBP1

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Specificity 

No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4.

Tested applications 
(see key)

ELISA, Neut, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 2 µg/ml. In the ELISA capture assay, plates are coated with 100 µl/well of the capture antibody (2 µg/ml) in combination with 100 µl/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained.
Neut: Use at a concentration of 10 - 40 µg/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 µg/ml in the presence of approximately 5 µg/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other

Relevance 

Insulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner. Post translational modification of IGFBPs, including phosphorylation and proteolysis, will modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions. IGFBP1 is expressed in liver, decidua, and kidneys and is the major IGF binding protein in human amnionic fluid. In hepatocytes, IGFBP1 production is regulated at the transcriptional level due to the effects of insulin and corticosteriods. IGFBP1 is the major determinant of the level of free IGF in serum. The expression of IGFBP1 is inhibited by insulin, IGFI, and IGFII and is stimulated by glucocorticoids, thyroid hormone, and epidermal growth factor (EGF), indicating an endocrine function. IGFBP1 shows inhibitory actions on cell proliferation and differentiation, presumably by interfering with the interactions between IGF and the IGF receptor (IGFR).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

33627.11

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for IGFBP1 antibody [33627.11] (ab10732)

  This product has been used in:

Walker G et al. Insulin-like growth factor binding proteins IGFBP3, IGFBP4, and IGFBP5 predict endocrine responsiveness in patients with ovarian cancer. Clin Cancer Res 13:1438-44 (2007). PubMed: 17332286

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