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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
Anti-IGFBP1 antibody
See all IGFBP1 products (7) ...
Description
Rabbit polyclonal to IGFBP1
Specificity
Recognizes mouse IGFBP 1 but not IGFBP 2, 3, 4, 5 or 6.
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Full length native protein (purified) from rat H-4-II-E Hepatoma cells.
Positive control
WB and IP: concentrated conditioned media from rat H-4-II-E hepatoma cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
10mM PBS pH 7.4 and 15mM sodium azide
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
WB
Application notes
IP: Use at a concentration of 5 µg/ml. 5µg immunoprecipitates IGFBP 1 from a 1:100 dilution of 10X conditioned media from rat H-4-II-E hepatoma cells.
WB: Use at a concentration of 1 µg/ml. 1µg/ml detects IGFBP 1 in a 1:10 dilution of 10X concentrated conditioned media from rat H-4-II-E hepatoma cells. 10X concentrated H-4-II-E conditioned media was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rat IGFBP 1 (1µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 32kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Post-translational
modifications
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
Cellular localization
Secreted.
Target information above from: UniProt accessionP08833
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AFBP antibody
- Alpha pregnancy associated endometrial globulin antibody
- Amniotic fluid binding protein antibody
see all
Database links
- Entrez Gene: 16006 Mouse
- Entrez Gene: 25685 Rat
- SwissProt: P47876 Mouse
- SwissProt: Q5SVY8 Mouse
- SwissProt: Q91WV8 Mouse
- SwissProt: P21743 Rat
- Unigene: 21300 Mouse
- Unigene: 34026 Rat
Protocols
References for Anti-IGFBP1 antibody (ab4242)
ab4242 has not yet been referenced specifically in any publications.
Publishing research using ab4242? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"